Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Heat shock protein beta-2 (HSPB2) Standard Curve.)
Rat Heat Shock Protein beta-2 (HSPB2) ELISA Kit | HSPB2 elisa kit
Rat Heat Shock Protein beta-2 (HSPB2) ELISA Kit
Reactivity
Rat
Synonyms
Heat Shock Protein beta-2 (HSPB2); Rat Heat Shock Protein beta-2 (HSPB2) ELISA Kit; HSPB2; HSP27; Hs.78846; LOH11CR1K; MGC133245; MKBP; heat shock 27kD protein 2; heat-shock protein beta-2; HSPB2 elisa kit
Reactivity
Rat
Specificity
Rat Heat shock protein beta-2 (HSPB2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat HSPB2. No significant cross-reactivity or interference between Rat HSPB2 and analogues was observed.
Sequence Length
182
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Heat shock protein beta-2 (HSPB2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Heat shock protein beta-2 (HSPB2) Standard Curve.)
Related Product Information for HSPB2 elisa kit
Description: This Rat Heat shock protein beta-2 (HSPB2) ELISA Kit employs a two-site sandwich ELISA to quantitate HSPB2 in samples. An antibody specific for HSPB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Heat shock protein beta-2 is a protein encoded by the HSPB2 gene.Alpha-B-crystallin (CRYAB) is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al. (1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al. (1997) also determined the complete genomic structure of the rat homolog of HSPB2.
Background: Heat shock protein beta-2 is a protein encoded by the HSPB2 gene.Alpha-B-crystallin (CRYAB) is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al. (1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al. (1997) also determined the complete genomic structure of the rat homolog of HSPB2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
heat shock protein beta-2
NCBI Official Synonym Full Names
heat shock protein family B (small) member 2
NCBI Official Symbol
Hspb2
NCBI Protein Information
heat shock protein beta-2
UniProt Protein Name
Heat shock protein beta-2
Protein Family
UniProt Gene Name
Hspb2
UniProt Synonym Gene Names
HspB2
NCBI Description
a small heat shock protein [RGD, Feb 2006]
Uniprot Description
May regulate the kinase DMPK.
Research Articles on HSPB2
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Product Notes
The Rat HSPB2 hspb2 (Catalog #AAA9712514) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712514 ELISA Kit recognizes Rat HSPB2. It is sometimes possible for the material contained within the vial of "Heat Shock Protein beta-2 (HSPB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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