Rabbit RNF34 Polyclonal Antibody | anti-RNF34 antibody

RNF34 Antibody

Cat. Num. AAA9630906

• Gene Names: RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RNF34; Polyclonal Antibody; RNF34 Antibody; CARP 1; CARP-1; CARP1; Caspase regulator CARP1; Caspases-8 and -10-associated RING finger protein 1; E3 ubiquitin-protein ligase RNF34; FYVE-RING finger protein Momo; hRFI; Human RING finger homologous to inhibitor of apoptosis protein; RFI; RIF; RIFF; RING finger protein 34; RING finger protein RIFF; RNF34_HUMAN; anti-RNF34 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RNF34 Antibody detects endogenous levels of RNF34.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RNF34 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RNF34.
• Conjugation: Unconjugated
• Similarity: The RING-type zinc finger is required for the ubiquitination of target proteins. The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids.
• Post Translational Modifications: Autoubiquitinated (in vitro).Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
• Subunit Structure: Interacts with CASP8 and CASP10. Interacts (via RING-type zinc finger) with PPARGC1A. Interacts with NOD1. Interacts with p53/TP53; involved in p53/TP53 ubiquitination. Interacts (via RING-type zinc finger) with MDM2; the interaction stabilizes MDM2.
• Tissue Specificity: Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
• Function: E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells. Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RNF34 antibody

Cell membrane; Peripheral membrane protein; Endomembrane system; Nucleus; Nucleus speckle; Cytoplasm; Cytosol; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 378925642
• NCBI GeneID: 80196
• NCBI Accession #: NP_001243787.1
• NCBI GenBank Nucleotide #: NM_001256858.1
• UniProt Accession #: Q969K3; Q8NG47; Q9H6W8; B7Z933
• Molecular Weight: 41,797 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF34 isoform 3
• NCBI Official Synonym Full Names: ring finger protein 34, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF34
• NCBI Official Synonym Symbols: RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF34; FYVE-RING finger protein MOMO; RING finger protein RIFF; caspase regulator CARP1; caspases-8 and -10-associated RING finger protein 1; human RING finger homologous to inhibitor of apoptosis protein
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF34
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF34
• UniProt Entry Name: RNF34_HUMAN

NCBI Description

The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

RNF34: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3- phosphate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cytoplasm; plasma membrane; endomembrane system; nuclear speck; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination