Western Blot (WB)
(RNF34 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RNF34 antibody detected the RNF34 protein (arrow).)
Rabbit RNF34 Polyclonal Antibody | anti-RNF34 antibody
RNF34 Antibody (C-term)
Western Blot (WB)
(RNF34 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RNF34 antibody detected the RNF34 protein (arrow).)
Western Blot (WB)
(RNF34 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RNF34 antibody detected the RNF34 protein (arrow).)
motif known to be involved in protein-protein and protein-DNA
interactions. This protein interacts with DNAJA3/hTid-1, which is a
DnaJ protein reported to function as a modulator of apoptosis.
Overexpression of this gene in Hela cells was shown to confer the
resistance to TNF-alpha induced apoptosis, suggesting an
anti-apoptotic function of this protein. This protein can be
cleaved by caspase-3 during the induction of apoptosis.
Alternatively spliced transcript variants encoding distinct
isoforms have been reported.
Yang, W., et al. Cell Cycle 7(5):670-682(2008)
Yang, W., et al. J. Biol. Chem. 282(5):3273-3281(2007)
Konishi, T., et al. Mol. Cancer Ther. 4(5):743-750(2005)
Sasaki, S., et al. J. Exp. Clin. Cancer Res. 23(3):507-512(2004)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
Uniprot Description
RNF34: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3- phosphate. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Ligase; Ubiquitin ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: cytoplasm; plasma membrane; endomembrane system; nuclear speck; cytosol; nucleus
Molecular Function: protein binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ligase activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination
Research Articles on RNF34
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Product Notes
The RNF34 rnf34 (Catalog #AAA9203074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 283-311. The RNF34 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RNF34 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the RNF34 rnf34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF34, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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