Rabbit anti-Human, Mouse WDFY3 Polyclonal Antibody | anti-WDFY3 antibody

WDFY3 Antibody

Cat. Num. AAA9630048

• Gene Names: WDFY3; ALFY; ZFYVE25
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: WDFY3; Polyclonal Antibody; WDFY3 Antibody; Alfy; Autophagy linked FYVE protein; KIAA0993; MGC16461; WD repeat and FYVE domain containing 3; ZFYVE25; anti-WDFY3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: WDFY3 Antibody detects endogenous levels of WDFY3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-WDFY3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human WDFY3.
• Conjugation: Unconjugated
• Similarity: The LIR (LC3-interacting region) motif mediates the interaction with MAP1LC3C and other ATG8 family members. The FYVE domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns3P).
• Subunit Structure: Directly interacts with ATG5 and associates with the ATG12-ATG5-ATG16L complex. Interacts with p62/SQSTM1; this interaction is required to recruit WDFY3 to cytoplasmic bodies and to PML bodies. Directly interacts with GABARAP, GABARAPL1 and GABARAPL2; the interaction with GABARAP is required for WDFY3 recruitment to MAP1LC3B-positive p62/SQSTM1 bodies. Weakly interacts with MAP1LC3C; this interaction is direct. Does not interact with MAP1LC3A, nor MAP1LC3B. Interacts with TRAF6 (By similarity).
• Tissue Specificity: Expressed in osteoclast and their mononuclear precursors (at protein level).
• Function: Required for selective macroautophagy (aggrephagy). Acts as an adapter protein by linking specific proteins destined for degradation to the core autophagic machinery members, such as the ATG5-ATG12-ATG16L E3-like ligase, SQSTM1 and LC3. Along with p62/SQSTM1, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with SQSTM1, required to recruit ubiquitinated proteins to PML bodies in the nucleus. Important for normal brain development. Essential for the formation of axonal tracts throughout the brain and spinal cord, including the formation of the major forebrain commissures. Involved in the ability of neural cells to respond to guidance cues. Required for cortical neurons to respond to the trophic effects of netrin-1/NTN1 (By similarity). Regulates Wnt signaling through the removal of DVL3 aggregates, likely in an autophagy-dependent manner. This process may be important for the determination of brain size during embryonic development. May regulate osteoclastogenesis by acting on the TNFSF11/RANKL - TRAF6 pathway (By similarity). After cytokinetic abscission, involved in midbody remnant degradation. In vitro strongly binds to phosphatidylinositol 3-phosphate (PtdIns3P).
• Note: Relocalization from the nucleus to the cytosol is stimulated by cellular stress, such as starvation or proteasomal inhibition. In the cytosol of starved cells, colocalizes with autophagic structures (PubMed:15292400, PubMed:20168092, PubMed:20971078, PubMed:20417604). This redistribution is dependent on p62/SQSTM1 (PubMed:20168092). When nuclear export is blocked by treatment with leptomycin B, accumulates in nuclear bodies, that completely or partially colocalize with promyelocytic leukemia (PML) bodies (PubMed:20168092). Localizes throughout neurons, including within axons. In neurons, enriched in the light membrane fraction along with the synaptosomal membrane protein synaptophysin and the membrane-bound form of LC3/MAP1LC3A/MAP1LC3B, called LC3-II, a classic marker for autophagic vesicles (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-WDFY3 antibody

Nucleus membrane; Cytoplasm; Cytosol; Nucleus; PML body; Membrane; Peripheral membrane protein; Cytoplasmic side; Perikaryon; Cell projection; Axon; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 31317272
• NCBI GeneID: 23001
• NCBI Accession #: NP_055806.2
• NCBI GenBank Nucleotide #: NM_014991.4
• UniProt Accession #: Q8IZQ1; Q4W5K5; Q6P0Q5; Q8N1T2; Q8NAV6; Q96BS7; Q96D33; Q96N85; Q9Y2J7
• Molecular Weight: 393,367 Da
• NCBI Official Full Name: WD repeat and FYVE domain-containing protein 3
• NCBI Official Synonym Full Names: WD repeat and FYVE domain containing 3
• NCBI Official Symbol: WDFY3
• NCBI Official Synonym Symbols: ALFY; ZFYVE25
• NCBI Protein Information: WD repeat and FYVE domain-containing protein 3
• UniProt Protein Name: WD repeat and FYVE domain-containing protein 3
• Protein Family: WD repeat and FYVE domain-containing protein
• UniProt Gene Name: WDFY3
• UniProt Synonym Gene Names: KIAA0993; Alfy

NCBI Description

This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy. [provided by RefSeq, May 2010]

Uniprot Description

Required for selective macroautophagy (aggrephagy). Acts as an adapter protein by linking specific proteins destined for degradation to the core autophagic machinery members, such as the ATG5-ATG12-ATG16L E3-like ligase, SQSTM1 and LC3 (PubMed:20417604). Along with p62/SQSTM1, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with SQSTM1, required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed:20168092). Important for normal brain development. Essential for the formation of axonal tracts throughout the brain and spinal cord, including the formation of the major forebrain commissures. Involved in the ability of neural cells to respond to guidance cues. Required for cortical neurons to respond to the trophic effects of netrin-1/NTN1 (). Regulates Wnt signaling through the removal of DVL3 aggregates, likely in an autophagy-dependent manner. This process may be important for the determination of brain size during embryonic development (PubMed:27008544). May regulate osteoclastogenesis by acting on the TNFSF11/RANKL - TRAF6 pathway (). After cytokinetic abscission, involved in midbody remnant degradation (PubMed:24128730). In vitro strongly binds to phosphatidylinositol 3-phosphate (PtdIns3P) (PubMed:15292400).