Goat Alfy/WDFY3 Polyclonal Antibody | anti-WDFY3 antibody

Goat anti-Alfy/WDFY3 Antibody

Cat. Num. AAA423300

• Gene Names: WDFY3; ALFY; ZFYVE25
• Reactivity: Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Alfy/WDFY3; Polyclonal Antibody; Goat anti-Alfy/WDFY3 Antibody; WDFY3; WD repeat and FYVE domain containing 3; ALFY; ZFYVE25; WD repeat and FYVE domain-containing protein 3; autophagy-linked FYVE protein; WDFY3 antibody; WD repeat and FYVE domain containing 3 antibody; ALFY antibody; ZFYVE25 antibody; WD repeat and FYVE domain-containing protein 3 antibody; autophagy-linked FYVE protein antibody; Alfy / WDFY3; anti-WDFY3 antibody

• Host: Goat
• Reactivity: Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SPERSTRTQQKEFQT
• Sequence Length: 3526

• Applicable Applications for anti-WDFY3 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 400kDa band observed in Mouse Brain lysates (calculated MW of 395kDa according to NP_055806.2). Data obtained from anonymous customer. The lower molecular weight bands are non-specific. We call for caution when used for other assays than Western blot. Recommended concentration: 4-6ug/ml.
• Immunogen: Peptide with sequence C-SPERSTRTQQKEFQT, from the internal region of the protein sequence according to NP_055806.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((5ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 31317272
• NCBI GeneID: 23001
• NCBI Accession #: NP_055806.2
• NCBI GenBank Nucleotide #: NM_014991.4
• Molecular Weight: 393,367 Da
• NCBI Official Full Name: WD repeat and FYVE domain-containing protein 3
• NCBI Official Synonym Full Names: WD repeat and FYVE domain containing 3
• NCBI Official Symbol: WDFY3
• NCBI Official Synonym Symbols: ALFY; ZFYVE25
• NCBI Protein Information: WD repeat and FYVE domain-containing protein 3
• UniProt Protein Name: WD repeat and FYVE domain-containing protein 3
• Protein Family: WD repeat and FYVE domain-containing protein
• UniProt Gene Name: WDFY3
• UniProt Synonym Gene Names: KIAA0993; Alfy
• UniProt Entry Name: WDFY3_HUMAN

NCBI Description

This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy. [provided by RefSeq, May 2010]

Uniprot Description

WDFY3: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, peripheral; Transferase; Lipid-binding; Nuclear envelope

Chromosomal Location of Human Ortholog: 4q21.23

Cellular Component: autophagic vacuole; cytoplasm; extrinsic to membrane; inclusion body; nuclear envelope; nuclear membrane; PML body

Molecular Function: beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; metal ion binding; phosphatidylinositol binding; protein binding

Biological Process: metabolic process; positive regulation of macroautophagy