Rabbit CREB3 Polyclonal Antibody | anti-CREB3 antibody

CREB3 Antibody

Cat. Num. AAA9629081

• Gene Names: CREB3; LZIP; LUMAN; sLZIP
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CREB3; Polyclonal Antibody; CREB3 Antibody; Basic leucine zipper protein; cAMP responsive element binding protein 3; Clic AMP responsive element binding protein 3; CREB 3; cyclic AMP response element (CRE) binding protein/activating transcription factor 1; Cyclic AMP responsive element binding protein 3; Leucine zipper protein; LUMAN; LZIP; MGC15333; MGC19782; Transcription factor LZIP alpha; anti-CREB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CREB3 Antibody detects endogenous levels of total CREB3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CREB3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the bZIP family. ATF subfamily.
• Post Translational Modifications: First proteolytically cleaved by site-1 protease (S1P) that generates membrane-associated N-terminus and a luminal C-terminus forms. The membrane-associated N-terminus form is further proteolytically processed probably by the site-2 protease (S2P) through a regulated intramembrane proteolysis (RIP), releasing the transcriptional active processed cyclic AMP-responsive element-binding protein 3 form, which is transported to the nucleus. The proteolytic cleavage is strongly induced during dendritic cell (DC) maturation and inhibited by DCSTAMP. That form is rapidly degraded.N-glycosylated.
• Subunit Structure: Homodimer. Isoform 1 interacts with HCFC1; the interaction is required to stimulate CREB3 transcriptional activity. Isoform 1 interacts with CREBZF; the interaction occurs only in combination with HCFC1. Isoform 1 interacts (via central part and transmembrane region) with DCSTAMP (via C-terminus cytoplasmic domain). Isoform 1 interacts with OS9. Isoform 1 interacts (via leucine-zipper domain) with CREBRF (via leucine-zipper domain); the interaction occurs only after CREB3 activation and promotes CREB3 degradation. Isoform 1 interacts (via C-terminal domain) with CCR1.(Microbial infection) Interacts with the HCV core protein; homodimerization is prevented by the HCV core protein. Isoform 1 interacts (via leucine-zipper and transmembrane domains) with HIV-1 TMgp41 (via cytoplasmic domain); the interaction reduces CREB3 stability. Processed cyclic AMP-responsive element-binding protein 3 interacts with HIV-1 Tat.
• Tissue Specificity: Ubiquitously expressed. Expressed in dendritic cells (DC). Weakly expressed in monocytes (at protein level).
• Function: Endoplasmic reticulum (ER)-bound sequence-specific transcription factor that directly binds DNA and activates transcription. Plays a role in the unfolded protein response (UPR), promoting cell survival versus ER stress-induced apoptotic cell death. Also involved in cell proliferation, migration and differentiation, tumor suppression and inflammatory gene expression. Acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration. Associates with chromatin to the HERPUD1 promoter. Also induces transcriptional activation of chemokine receptors.This is the transcriptionally active form that translocates to the nucleus and activates unfolded protein response (UPR) target genes during endoplasmic reticulum (ER) stress response. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3') and C/EBP sequences present in many promoters to activate transcription of the genes. Binds to the unfolded protein response element (UPRE) consensus sequences sites. Binds DNA to the 5'-CCAC[GA]-3'half of ERSE II (5'-ATTGG-N-CCACG-3').Functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases (HDAC1, HDAC2 and HDAC6). Also decreases the acetylation level of histone H4. Does not promote the chemotactic activity of leukocyte cells.(Microbial infection) Plays a role in human immunodeficiency virus type 1 (HIV-1) virus protein expression.(Microbial infection) Plays a role in herpes simplex virus-1 (HSV-1) latent infection and reactivation from latency. Represses the VP16-mediated transactivation of immediate early genes of the HSV-1 virus by sequestering host cell factor-1 HCFC1 in the ER membrane of sensory neurons, thereby preventing the initiation of the replicative cascade leading to latent infection.(Microbial infection) May play a role as a cellular tumor suppressor that is targeted by the hepatitis C virus (HCV) core protein.(Microbial infection) Activates transcription of genes required for reactivation of the latent HSV-1 virus. It's transcriptional activity is inhibited by CREBZF in a HCFC1-dependent manner, by the viral transactivator protein VP16. Binds DNA to the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3') and C/EBP sequences present in many viral promoters.(Microbial infection) It's transcriptional activity is inhibited by CREBZF in a HCFC1-dependent manner, by the viral transactivator HCV core protein.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CREB3 antibody

Endoplasmic reticulum membrane; Single-pass type II membrane protein; Golgi apparatus; Primary; Nucleus; Cytoplasm

NCBI and Uniprot Product Information

• NCBI GI #: 38327637
• NCBI GeneID: 10488
• NCBI Accession #: NP_006359.3
• NCBI GenBank Nucleotide #: NM_006368
• UniProt Accession #: O43889; O14671; O14919; Q5TCV1; Q96GK8; Q9H2W3; Q9UE77; D0PTW6
• Molecular Weight: 39,580 Da
• NCBI Official Full Name: Homo sapiens cAMP responsive element binding protein 3 (CREB3), mRNA
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 3
• NCBI Official Symbol: CREB3
• NCBI Official Synonym Symbols: LZIP; LUMAN; sLZIP
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 3; CREB-3; basic leucine zipper protein; cAMP-responsive element-binding protein 3; cyclic AMP response element (CRE)-binding protein/activating transcription factor 1; leucin zipper proitein; small leucine zi
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 3
• Protein Family: CREB3 regulatory factor
• UniProt Gene Name: CREB3
• UniProt Synonym Gene Names: LZIP; CREB-3; cAMP-responsive element-binding protein 3; N-terminal Luman; Transcriptionally active form
• UniProt Entry Name: CREB3_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-response element and regulates cell proliferation. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. This protein also plays a role in leukocyte migration, tumor suppression, and endoplasmic reticulum stress-associated protein degradation. Additional transcript variants have been identified, but their biological validity has not been determined.[provided by RefSeq, Nov 2009]

Uniprot Description

CREB3: Endoplasmic reticulum (ER)-bound transcription factor that plays a role in the unfolded protein response (UPR). Involved in cell proliferation and migration, tumor suppression and inflammatory gene expression. Plays also a role in the human immunodeficiency virus type 1 (HIV-1) virus protein expression and in the herpes simplex virus-1 (HSV-1) latent infection and reactivation from latency. Isoform 2 plays a role in the unfolded protein response (UPR). Isoform 2 acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration. Isoform 2 may play a role as a cellular tumor suppressor that is targeted by the hepatitis C virus (HSV) core protein. Isoform 2 represses the VP16-mediated transactivation of immediate early genes of the HSV-1 virus by sequestring host cell factor-1 HCFC1 in the ER membrane of sensory neurons, thereby preventing the initiation of the replicative cascade leading to latent infection. Isoform 3 functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases (HDAC1, HDAC2 and HDAC6). Isoform 3 decreases the acetylation level of histone H4. Isoform 3 does not promote the chemotactic activity of leukocyte cells. Belongs to the bZIP family. ATF subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: Golgi membrane; Golgi apparatus; nuclear body; endoplasmic reticulum membrane; membrane; cell soma; endoplasmic reticulum; cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; cytosol; nucleus

Molecular Function: protein dimerization activity; CCR1 chemokine receptor binding; protein binding; protein homodimerization activity; DNA binding; cAMP response element binding protein binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cytoplasmic sequestering of transcription factor; viral reproduction; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription of target genes involved in unfolded protein response; establishment of viral latency; chemotaxis; induction of positive chemotaxis; negative regulation of cell cycle; regulation of cell proliferation; reactivation of latent virus; positive regulation of transcription from RNA polymerase II promoter; regulation of cell growth; positive regulation of calcium ion transport; positive regulation of defense response to virus by host; positive regulation of cell migration