Goat SIRT1 / Sirtuin 1 Polyclonal Antibody | anti-SIRT1 antibody

Goat Polyclonal to Human SIRT1 / Sirtuin 1

Cat. Num. AAA243883

• Gene Names: SIRT1; SIR2L1
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SIRT1 / Sirtuin 1; Polyclonal Antibody; Goat Polyclonal to Human SIRT1 / Sirtuin 1; Anti-SIRT1 / Sirtuin 1 Antibody (Internal) IHC-plus; SIRT1; SIR2-like protein 1; SIR2alpha; SIR2L1; Sirtuin 1; Sir2-like 1; Sirtuin type 1; HSIR2; HSIRT1; Human SIRT1; anti-SIRT1 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human SIRT1. This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 747

• Applicable Applications for anti-SIRT1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (3.75 ug/ml), WB (0.05 - 0.2 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 120kD band observed in lysates of cell line Jurkat (calculated MW of 81.7kD according to NP_036370.2). The observed molecular weight corresponds to earlier findings in lit...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-EKPQEVQTSRNVES from an internal region of human SIRT1 (NP_036370.2; NP_001135970.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse (93%); Rat (86%).
• Immunogen Type: Synthetic peptide
• Immunogen: SIRT1 / Sirtuin 1 antibody was raised against synthetic peptide C-EKPQEVQTSRNVES from an internal region of human SIRT1 (NP_036370.2; NP_001135970.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse (93%); Rat (86%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SIRT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)

Western Blot (WB)

(SIRT1 antibody (0.05 ug/ml) staining of Jurkat lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-SIRT1 antibody

The SIRT family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes including transcriptional regulation, cell cycle progression, DNA damage repair and aging. Sirtuins utilize NAD+ as a substrate to catalyze deacetylation of specific acetylated protein and histones. SIRT1 has been shown to be induced by caloric restriction (CR) in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR.

Product Categories/Family for anti-SIRT1 antibody

Family: ADP-Ribosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 7657575
• NCBI GeneID: 23411
• NCBI Accession #: NP_036370.2
• NCBI GenBank Nucleotide #: NM_012238.4
• UniProt Accession #: Q96EB6; Q2XNF6; Q5JVQ0; Q9GZR9; Q9Y6F0
• Molecular Weight: 61,066 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-1 isoform a
• NCBI Official Synonym Full Names: sirtuin 1
• NCBI Official Symbol: SIRT1
• NCBI Official Synonym Symbols: SIR2L1
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-1
• Protein Family: NAD-dependent protein deacetylase sirtuin
• UniProt Gene Name: SIRT1
• UniProt Synonym Gene Names: SIR2L1; hSIRT1; hSIR2; 75SirT1
• UniProt Entry Name: SIR1_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Uniprot Description

SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Apoptosis; EC 3.5.1.-; Deacetylase

Chromosomal Location of Human Ortholog: 10q21.3

Cellular Component: nucleoplasm; chromatin silencing complex; PML body; mitochondrion; ESC/E(Z) complex; nuclear chromatin; cytoplasm; nucleolus; nuclear inner membrane; nuclear envelope; nuclear heterochromatin; nucleus

Molecular Function: deacetylase activity; protein C-terminus binding; identical protein binding; histone binding; p53 binding; metal ion binding; protein deacetylase activity; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity; bHLH transcription factor binding; mitogen-activated protein kinase binding; histone deacetylase activity; HLH domain binding; transcription corepressor activity; NAD+ ADP-ribosyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; muscle development; viral reproduction; positive regulation of apoptosis; regulation of mitotic cell cycle; positive regulation of caspase activity; protein ubiquitination; negative regulation of prostaglandin biosynthetic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of gene expression, epigenetic; behavioral response to starvation; positive regulation of adaptive immune response; positive regulation of histone H3-K9 methylation; positive regulation of DNA repair; transcription, DNA-dependent; negative regulation of transcription factor activity; pyrimidine dimer repair via nucleotide-excision repair; cell aging; negative regulation of I-kappaB kinase/NF-kappaB cascade; ovulation from ovarian follicle; negative regulation of fat cell differentiation; cellular response to starvation; protein amino acid ADP-ribosylation; cholesterol homeostasis; regulation of endodeoxyribonuclease activity; methylation-dependent chromatin silencing; inhibition of NF-kappaB transcription factor; maintenance of chromatin silencing; negative regulation of phosphorylation; regulation of protein import into nucleus, translocation; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; triacylglycerol mobilization; negative regulation of transcription, DNA-dependent; rRNA processing; peptidyl-lysine acetylation; negative regulation of apoptosis; chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; establishment of chromatin silencing; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; response to insulin stimulus; DNA synthesis during DNA repair; protein amino acid deacetylation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; circadian regulation of gene expression; angiogenesis; DNA replication; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of chromatin silencing; single strand break repair; negative regulation of TOR signaling pathway; negative regulation of protein kinase B signaling cascade; protein destabilization; histone deacetylation; DNA repair; regulation of cell proliferation; negative regulation of helicase activity; response to hydrogen peroxide; fatty acid homeostasis; white fat cell differentiation; cell glucose homeostasis; spermatogenesis; gene expression; negative regulation of cell growth; positive regulation of insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; response to DNA damage stimulus; positive regulation of macroautophagy