TLS/FUS blocking peptide

TLS/FUS Blocking Peptide

Cat. Num. AAA9627472

• Gene Names: FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: TLS/FUS; TLS/FUS Blocking Peptide; 75 kDa DNA pairing protein; 75 kDa DNA-pairing protein; ALS6; Amyotrophic lateral sclerosis 6; fus; FUS CHOP; Fus like protein; FUS_HUMAN; FUS1; Fused in sarcoma; Fusion (involved in t(12; 16) in malignant liposarcoma); Fusion derived from t(12; 16) malignant liposarcoma; Fusion gene in myxoid liposarcoma; Heterogeneous nuclear ribonucleoprotein P2; hnRNP P2; hnRNPP2; Oncogene FUS; Oncogene TLS; POMp75; RNA binding protein FUS; RNA-binding protein FUS; TLS; TLS CHOP; Translocated in liposarcoma; Translocated in liposarcoma protein; TLS/FUS blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for TLS/FUS blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TLS/FUS blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 283135201
• NCBI GeneID: 2521
• NCBI Accession #: NP_001164105.1
• NCBI GenBank Nucleotide #: NM_001170634.1
• UniProt Accession #: P35637; Q9H4A8
• Molecular Weight: 53426 MW
• NCBI Official Full Name: RNA-binding protein FUS isoform 2
• NCBI Official Synonym Full Names: FUS RNA binding protein
• NCBI Official Symbol: FUS
• NCBI Official Synonym Symbols: TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• NCBI Protein Information: RNA-binding protein FUS
• UniProt Protein Name: RNA-binding protein FUS
• UniProt Gene Name: FUS
• UniProt Synonym Gene Names: TLS
• UniProt Entry Name: FUS_HUMAN

NCBI Description

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]

Uniprot Description

hnRNP P2: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single- stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP. Ubiquitous. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding; Nuclear receptor co-regulator; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression

Disease: Amyotrophic Lateral Sclerosis 6, With Or Without Frontotemporal Dementia; Tremor, Hereditary Essential, 4