Rabbit FUS/TLS Polyclonal Antibody | anti-FUS antibody

FUS/TLS Polyclonal Antibody

Cat. Num. AAA3012726

• Gene Names: FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: FUS/TLS; Polyclonal Antibody; FUS/TLS Polyclonal Antibody; FUS; 75 kDa DNA pairing protein; 75 kDa DNA-pairing protein; ALS6; Amyotrophic lateral sclerosis 6; fus; FUS CHOP; Fus like protein; FUS1; Fused in sarcoma; Fusion (involved in t(12; 16) in malignant liposarcoma); Fusion derived from t(12; 16) malignant liposarcoma; Fusion gene in myxoid liposarcoma; Heterogeneous nuclear ribonucleoprotein P2; hnRNP P2; hnRNPP2; Oncogene FUS; Oncogene TLS; POMp75; RNA binding protein FUS; RNA-binding protein FUS; TLS; TLS CHOP; Translocated in liposarcoma; Translocated in liposarcoma protein; anti-FUS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FUS/TLS polyclonal antibody detects endogenous levels of FUS/TLS protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 526

• Applicable Applications for anti-FUS antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of FUS/TLS on K562 cells lysates using anti-FUS/TLS antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining FUS/TLS in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-FUS antibody

EWS and FUS/TLS are nuclear RNA-binding proteins. As a result of chromosome translocation, the EWS gene is fused to a variety of transcription factors, including ATF-1, in human neoplasias. In the Ewing family of tumors, the N-terminal domain of EWS is fused to the DNA-binding domain of various Ets transcription factors, including Fli-1, ETV1 and FEV. The EWS/Fli-1 chimeric protein acts as a more potent transcriptional activator than Fli-1 and can promote cell transformation. In human myxoid liposarcomas and myeloid leukemias, chromosomal translocation results in the fusion of the N-terminal region of FUS/TLS with the open reading frame of CHOP. In normal cells, FUS/TLS binds to the DNA-binding domains of nuclear steroid receptors and is also present in subpopulations of TFIID complexes, indicating a potential role for FUS/TLS in the processing of primary transcripts that are generated in response to hormone-induced transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 386157
• NCBI GeneID: 2521
• UniProt Accession #: P35637; P56959
• Molecular Weight: 75kDa
• NCBI Official Full Name: TLS
• NCBI Official Synonym Full Names: FUS RNA binding protein
• NCBI Official Symbol: FUS
• NCBI Official Synonym Symbols: TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• NCBI Protein Information: RNA-binding protein FUS
• UniProt Protein Name: RNA-binding protein FUS
• Protein Family: RNA-binding protein
• UniProt Gene Name: FUS
• UniProt Synonym Gene Names: TLS
• UniProt Entry Name: FUS_HUMAN

NCBI Description

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]

Uniprot Description

hnRNP P2: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single- stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP. Ubiquitous. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding; Nuclear receptor co-regulator; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression

Disease: Amyotrophic Lateral Sclerosis 6, With Or Without Frontotemporal Dementia; Tremor, Hereditary Essential, 4