Pyruvate carboxylase, mitochondrial (Pc) Recombinant Protein | Pc recombinant protein

Recombinant Rat Pyruvate carboxylase, mitochondrial (Pc) , partial

• Gene Names: Pc; Pcx
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Pyruvate carboxylase; mitochondrial (Pc); Recombinant Rat Pyruvate carboxylase; partial; Pc recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,178

• Species: Rat
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Pc recombinant protein

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5 UTRs, but encoding the same protein, have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 31543464
• NCBI GeneID: 25104
• NCBI Accession #: NP_036876.2
• NCBI GenBank Nucleotide #: NM_012744.2
• UniProt Accession #: P52873; Q5RKM0; Q64555
• Molecular Weight: 129,777 Da
• NCBI Official Full Name: pyruvate carboxylase, mitochondrial
• NCBI Official Synonym Full Names: pyruvate carboxylase
• NCBI Official Symbol: Pc
• NCBI Official Synonym Symbols: Pcx
• NCBI Protein Information: pyruvate carboxylase, mitochondrial
• UniProt Protein Name: Pyruvate carboxylase, mitochondrial
• UniProt Gene Name: Pc
• UniProt Synonym Gene Names: PCB

NCBI Description

biotin-containing enzyme that catalyses the carboxylation of pyruvate to form oxaloacetate [RGD, Feb 2006]

Uniprot Description

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.