Chemiluminescence
(Staining of Mouse Liver lysate (35ug protein in RIPA buffer) using MBS6120603. Primary incubation was 1 hour. Detected by chemiluminescence.)
anti-Human, mouse Pyruvate Carboxylase Polyclonal Antibody
Goat Anti-Pyruvate Carboxylase
Reactivity
Human, mouse
Applications
ELISA, Western Blot
Purity
Affinity Purified. Purified by immunoaffinity chromatography.
Synonyms
Pyruvate Carboxylase; Polyclonal Antibody; Goat Anti-Pyruvate Carboxylase; PC; PCB; Pyruvate carboxylase; mitochondrial; Pyruvic carboxylase; anti-Pyruvate Carboxylase antibody
Host
Host: Goat; Source: Mouse
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse PC at ~150kD. Species Crossreactivity:
Purity/Purification
Affinity Purified. Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Applicable Applications for anti-Pyruvate Carboxylase antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Suitable for use in Peptide ELISA and Western Blot. Peptide ELISA: 1:16,000 Western Blot: 0.03-0.1ug/ml
Positive Control
Mouse Liver lysates
Immunogen
Synthetic peptide, C-KFKEVKKAYVEANQ, from the internal region of human PC (NP_000911.2, NP_071504.2). Species Sequence Homology: rat, canine, bovine and porcine
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
(Staining of Mouse Liver lysate (35ug protein in RIPA buffer) using MBS6120603. Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
(Staining of Mouse Liver lysate (35ug protein in RIPA buffer) using MBS6120603. Primary incubation was 1 hour. Detected by chemiluminescence.)
Testing Data
(HEK293 overexpressing Human Pyruvate Carboxylase (RC221895) and probed with MBS6120603 (mock transfection in first lane), tested by Origene.)
Testing Data
(HEK293 overexpressing Human Pyruvate Carboxylase (RC221895) and probed with MBS6120603 (mock transfection in first lane), tested by Origene.)
Related Product Information for anti-Pyruvate Carboxylase antibody
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The Pyruvate Carboxylase (Catalog #AAA6120603) is an Antibody produced from Host: Goat; Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Pyruvate Carboxylase reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Pyruvate Carboxylase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Suitable for use in Peptide ELISA and Western Blot. Peptide ELISA: 1:16,000 Western Blot: 0.03-0.1ug/ml. Researchers should empirically determine the suitability of the Pyruvate Carboxylase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyruvate Carboxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
