Glyoxalase I Active Protein | GLO1 active protein

Human Recombinant Glyoxalase I

Cat. Num. AAA845142

• Gene Names: GLO1; GLYI; GLOD1; HEL-S-74
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: Glyoxalase I; Human Recombinant Glyoxalase I; Lactoylglutathione lyase; Glx 1; GLO-1 Methylglyoxalase; Aldoketomutase; GLO1 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM
• Sequence Length: 184

• Applicable Applications for GLO1 active protein: SDS-PAGE
• Biological Activity: Specific activity: >0.4 units/mg.
• Results: >0.4 units/mg
• Unit Definition: One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25 degree C. Specific activity was expressed in units/mg of protein.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for GLO1 active protein

Background: GLO1 is involved in the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. GLO1 is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. GLO1 enzyme is abundantly expressed and present in numerous tumor cell lines, in which its concentration is often upregulated ubiquitously. GLO1 is a major susceptible gene for autism in an ethnic Chinese population from Taiwan. GLO1 might be involved in the pathophysiology of mood disorders. GLO1 plays a role in the pathophysiology of mood disorders. Overexpression of GLO1 is associated with kidney tumor.

Product Categories/Family for GLO1 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 118402586
• NCBI GeneID: 2739
• NCBI Accession #: NP_006699.2
• NCBI GenBank Nucleotide #: NM_006708.2
• UniProt Accession #: Q04760; P78375; Q59EL0; Q5TZW3; Q96FC0; Q96J41; B2R6P7; B4DDV0
• Molecular Weight: 20.7 kDa (184 aa, 1-184 aa)
• NCBI Official Full Name: lactoylglutathione lyase
• NCBI Official Synonym Full Names: glyoxalase I
• NCBI Official Symbol: GLO1
• NCBI Official Synonym Symbols: GLYI; GLOD1; HEL-S-74
• NCBI Protein Information: lactoylglutathione lyase
• UniProt Protein Name: Lactoylglutathione lyase
• UniProt Gene Name: GLO1
• UniProt Synonym Gene Names: Glx I
• UniProt Entry Name: LGUL_HUMAN

NCBI Description

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]

Uniprot Description

GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Carbohydrate Metabolism - pyruvate; EC 4.4.1.5

Chromosomal Location of Human Ortholog: 6p21.3-p21.1

Cellular Component: cytoplasm

Molecular Function: zinc ion binding; lactoylglutathione lyase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; glutathione metabolic process; carbohydrate metabolic process; osteoclast differentiation; negative regulation of apoptosis; methylglyoxal metabolic process

Disease: Autism