Western Blot (WB)
(Western blot analysis NHE-3 (Phospho-Ser555) using EGF treated A431 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit NHE-3 Polyclonal Antibody | anti-NHE-3 antibody
Phospho-NHE-3 (Ser555) Antibody
Gene Names
SLC9A3; NHE3; DIAR8; NHE-3
Reactivity
Human, RatPredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
NHE-3; Polyclonal Antibody; Phospho-NHE-3 (Ser555) Antibody; MGC126718; MGC126720; Na (+)/H (+) exchanger 3; NHE 3; NHE3; SL9A3_HUMAN; SLC9A 3; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger; apical epithelial; Solute carrier family 9 (sodium/hydrogen exchanger); isoform 3; member 3; Solute carrier family 9 member 3; anti-NHE-3 antibody
Host
Rabbit
Reactivity
Human, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-NHE-3 (Ser555) Antibody detects endogenous levels of NHE-3 only when phosphorylated at Ser555.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NHE-3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human NHE-3 around the phosphorylation site of Ser555.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated by PKA, which inhibits activity. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane.
Subunit Structure
Binds SLC9A3R1 and SLC9A3R2. Interacts with CHP1, CHP2 and SHANK2. Interacts with PDZK1 (via C-terminal PDZ domain) (By similarity). Interacts with PDZD3 and interactions decrease in response to elevated calcium ion levels. Interacts with AHCYL1; the interaction is required for SLC9A3 activity.
Similarity
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Subcellular Location
Apical cell membrane>Multi-pass membrane protein.
Note: In intestinal epithelial cells, localizes to the ileal brush border. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane. Angiotensin-2 enhances apical expression (By similarity).
Note: In intestinal epithelial cells, localizes to the ileal brush border. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane. Angiotensin-2 enhances apical expression (By similarity).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis NHE-3 (Phospho-Ser555) using EGF treated A431 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis NHE-3 (Phospho-Ser555) using EGF treated A431 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Immunohistochemistry (IHC)
(Staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-NHE-3 antibody
Involved in pHregulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,904 Da
NCBI Official Full Name
sodium/hydrogen exchanger 3 isoform 2
NCBI Official Synonym Full Names
solute carrier family 9 member A3
NCBI Official Symbol
SLC9A3
NCBI Official Synonym Symbols
NHE3; DIAR8; NHE-3
NCBI Protein Information
sodium/hydrogen exchanger 3
UniProt Protein Name
Sodium/hydrogen exchanger 3
UniProt Gene Name
SLC9A3
UniProt Synonym Gene Names
NHE-3
NCBI Description
The protein encoded by this gene is an epithelial brush border Na/H exchanger that uses an inward sodium ion gradient to expel acids from the cell. Defects in this gene are a cause of congenital secretory sodium diarrhea. Pseudogenes of this gene exist on chromosomes 10 and 22. [provided by RefSeq, Mar 2016]
Uniprot Description
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient (PubMed:26358773). Plays an important role in signal transduction.
Research Articles on NHE-3
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Product Notes
The NHE-3 slc9a3 (Catalog #AAA9614488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-NHE-3 (Ser555) Antibody reacts with Human, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's NHE-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:1000-3000 IHC: 1:50-1:200 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the NHE-3 slc9a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NHE-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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