Rabbit JIP1 Polyclonal Antibody | anti-JIP1 antibody

JIP1 Antibody

Cat. Num. AAA9613833

• Gene Names: MAPK8IP1; IB1; JIP1; JIP-1; PRKM8IP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: JIP1; Polyclonal Antibody; JIP1 Antibody; C jun amino terminal kinase interacting protein 1; C-jun-amino-terminal kinase-interacting protein 1; IB 1; IB-1; IB1; Islet brain 1; Islet-brain 1; JIP 1; JIP-1; JIP1_HUMAN; JNK interacting protein 1; JNK MAP kinase scaffold protein 1; JNK-interacting protein 1; MAPK8IP 1; MAPK8IP1; MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1; Mitogen activated protein kinase 8 interacting protein 1; Mitogen-activated protein kinase 8-interacting protein 1; PRKM8; PRKM8 interacting protein; PRKM8IP; anti-JIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: JIP1 Antibody detects endogenous levels of total JIP1.; Tissue Specificity: Highly expressed in brain. Expressed in neurons, localizing to neurite tips in differentiating cells. Also expressed in the pancreas, testis and prostate. Low levels in heart, ovary and small intestine. Decreased levels in pancreatic beta cells sensitize cells to IL-1-beta-induced apoptosis.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-JIP1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: IF/ICC: 1:100-1:500; WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human JIP1.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated by MAPK8, MAPK9 and MAPK10. Phosphorylation on Thr-103 is also necessary for the dissociation and activation of MAP3K12. Phosphorylated by isoform 1 and isoform 2 of VRK2. Hyperphosphorylated during mitosis following activation of stress-activated and MAP kinases.Ubiquitinated. Two preliminary events are required to prime for ubiquitination; phosphorylation and an increased in intracellular calcium concentration. Then, the calcium influx initiates ubiquitination and degradation by the ubiquitin-proteasome pathway.
• Subunit Structure: Forms homo- or heterooligomeric complexes. Binds specific components of the JNK signaling pathway namely, MAPK8/JNK1, MAPK9/JNK2, MAPK10/JNK3, MAP2K7/MKK7, MAP3K11/MLK3 and DLK1. Also binds the proline-rich domain-containing splice variant of apolipoprotein E receptor 2 (ApoER2). Interacts, via the PID domain, with ARHGEF28. Binds the cytoplasmic tails of LRP1 and LRP2 (Megalin). Binds the TPR motif-containing C-terminal of KNS2, then the pre-assembled MAPK8IP1 scaffolding complexes are transported as a cargo of kinesin, to the required subcellular location. Interacts with the cytoplasmic domain of APP. Interacts with DCLK2 (By similarity). Interacts with MAP3K7/TAK1. Interacts with isoform 1 and isoform 2 of VRK2. Found in a complex with SH3RF1, RAC1, MAP3K11/MLK3, MAP2K7/MKK7 and MAPK8/JNK1. Found in a complex with SH3RF1, RAC2, MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and MAPK9/JNK2. Interacts with SH3RF2 (By similarity).
• Similarity: The destruction boxes (D-box) may act as recognition signals for degradation via the ubiquitin-proteasome pathway.A minimal inhibitory domain prevents pancreatic beta cell apoptosis in vitro, and prevents activation of c-jun by MAPK8, MAPK9 and MAPK10.The SH3 domain mediates homodimerization.Belongs to the JIP scaffold family.
• Subcellular Location: Cytoplasm. Cytoplasm>Perinuclear region. Nucleus. Endoplasmic reticulum membrane. Mitochondrion membrane.; Note: Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Rat brain, using JIP1 Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6812) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-JIP1 antibody

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response. Acts as a scaffold protein that coordinates with SH3RF1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the activation of MAPK8/JNK1 and differentiation of CD8 (+) T-cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4885433
• NCBI GeneID: 9479
• NCBI Accession #: NP_005447.1
• NCBI GenBank Nucleotide #: NM_005456.3
• UniProt Accession #: Q9UQF2; O43407; D3DQP4
• Molecular Weight: 77,524 Da
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 1
• NCBI Official Symbol: MAPK8IP1
• NCBI Official Synonym Symbols: IB1; JIP1; JIP-1; PRKM8IP
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 1; IB-1; islet-brain 1; JNK-interacting protein 1; PRKM8 interacting protein; JNK MAP kinase scaffold protein 1
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 1
• UniProt Gene Name: MAPK8IP1
• UniProt Synonym Gene Names: IB1; JIP1; PRKM8IP; JIP-1; JNK-interacting protein 1; IB-1
• UniProt Entry Name: JIP1_HUMAN