Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using FHOD1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit FHOD1 Polyclonal Antibody | anti-FHOD1 antibody
FHOD1 Antibody
Gene Names
FHOD1; FHOS
Reactivity
Human, MousePredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
FHOD1; Polyclonal Antibody; FHOD1 Antibody; FH1/FH2 domain containing protein; FH1/FH2 domain containing protein 1; FH1/FH2 domain-containing protein 1; Fhod1; FHOD1_HUMAN; FHOS; FHOS1; Formin homolog overexpressed in spleen 1; Formin homology 2 domain containing 1; Formin homology 2 domain containing protein 1; Formin homology 2 domain-containing protein 1; anti-FHOD1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(86%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(86%)
Clonality
Polyclonal
Isotype
IgG
Specificity
FHOD1 Antibody detects endogenous levels of total FHOD1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
1164
Applicable Applications for anti-FHOD1 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human FHOD1, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using FHOD1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using FHOD1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-FHOD1 antibody
Function: Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Acts synergistically with ROCK1 to promote SRC-dependent non-apoptotic plasma membrane blebbing.
Post Translational Modifications: Phosphorylated by ROCK1.
Subcellular Location: Cytoplasm. Cytoplasm>Cytoskeleton. Cell projection>Bleb. Note: Predominantly cytoplasmic.
Tissue Specificity: Ubiquitous. Highly expressed in spleen.
Subunit Structure: Self-associates via the FH2 domain. Binds to F-actin via its N-terminus. Binds to the cytoplasmic domain of CD21 via its C-terminus. Interacts with ROCK1 in a Src-dependent manner.
Similarity: The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.Belongs to the formin homology family.
Post Translational Modifications: Phosphorylated by ROCK1.
Subcellular Location: Cytoplasm. Cytoplasm>Cytoskeleton. Cell projection>Bleb. Note: Predominantly cytoplasmic.
Tissue Specificity: Ubiquitous. Highly expressed in spleen.
Subunit Structure: Self-associates via the FH2 domain. Binds to F-actin via its N-terminus. Binds to the cytoplasmic domain of CD21 via its C-terminus. Interacts with ROCK1 in a Src-dependent manner.
Similarity: The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.Belongs to the formin homology family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)126kD.
Predicted Molecular Weight: (Calculated)127kDa.
Predicted Molecular Weight: (Calculated)127kDa.
NCBI Official Full Name
FH1/FH2 domain-containing protein 1 isoform 2
NCBI Official Synonym Full Names
formin homology 2 domain containing 1
NCBI Official Symbol
FHOD1
NCBI Official Synonym Symbols
FHOS
NCBI Protein Information
FH1/FH2 domain-containing protein 1
UniProt Protein Name
FH1/FH2 domain-containing protein 1
Protein Family
UniProt Gene Name
FHOD1
UniProt Synonym Gene Names
FHOS; FHOS1; FHOS
UniProt Entry Name
FHOD1_HUMAN
NCBI Description
This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Research Articles on FHOD1
Similar Products
Product Notes
The FHOD1 fhod1 (Catalog #AAA9612475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FHOD1 Antibody reacts with Human, Mouse Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(86%) and may cross-react with other species as described in the data sheet. AAA Biotech's FHOD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the FHOD1 fhod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FHOD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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