Rabbit Ambra1 Polyclonal Antibody | anti-Ambra1 antibody

Ambra1 Antibody

Cat. Num. AAA9405871

• Gene Names: AMBRA1; DCAF3; WDR94
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Ambra1; Polyclonal Antibody; Ambra1 Antibody; Activating molecule in beclin-1-regulated autophagy; WDR94; anti-Ambra1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 1301

• Applicable Applications for anti-Ambra1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 15 amino acid peptide from near the carboxy terminus of human Ambra1.
• Target Name: Ambra1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Ambra1 in 3T3 cell lysate with Ambra1 antibody at 1 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Ambra1 in human brain with Ambra1 antibody at 5 ug/mL.)

Related Product Information for anti-Ambra1 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.

Product Categories/Family for anti-Ambra1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 392306969
• NCBI GeneID: 55626
• NCBI Accession #: NP_001254711.1
• NCBI GenBank Nucleotide #: NM_001267782.1
• UniProt Accession #: Q9C0C7; Q86XD6; Q9H8Z0; Q9NXE7; A6XN33; D3DQP8; G3V193
• Molecular Weight: 84,428 Da
• NCBI Official Full Name: activating molecule in BECN1-regulated autophagy protein 1 isoform 1
• NCBI Official Synonym Full Names: autophagy/beclin-1 regulator 1
• NCBI Official Symbol: AMBRA1
• NCBI Official Synonym Symbols: DCAF3; WDR94
• NCBI Protein Information: activating molecule in BECN1-regulated autophagy protein 1
• UniProt Protein Name: Activating molecule in BECN1-regulated autophagy protein 1
• Protein Family: Activating molecule in BECN1-regulated autophagy protein
• UniProt Gene Name: AMBRA1
• UniProt Synonym Gene Names: KIAA1736
• UniProt Entry Name: AMRA1_HUMAN

Uniprot Description

AMBRA1: a WD40 protein that regulates autophagy and development of the nervous system. A highly conserved vertebrate protein whose expression is mostly confined to the neuroepithelium during early neurogenesis. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. Interacts with ATG6. Forms a complex with ATG6 and PIK3C3. Its functional inactivation in mouse is lethal in utero (starting from embryonic day 14.5), leading to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, and excessive apoptosis. Hyperproliferation followed by a wave of caspase-dependent cell death was the earliest detectable abnormality in the developing neuroepithelium. Five isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Autophagy

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: mitochondrial outer membrane; mitochondrion; intracellular membrane-bound organelle; cytoplasm; autophagic vacuole; axoneme; phagocytic vesicle; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: neural tube development; negative regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; autophagy; negative regulation of neuron apoptosis; cell differentiation