Rabbit Repulsive Guidance Molecule A Polyclonal Antibody | anti-RGMA antibody

Repulsive Guidance Molecule A Antibody

Cat. Num. AAA9612381

• Gene Names: RGMA; RGM
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Repulsive Guidance Molecule A; Polyclonal Antibody; Repulsive Guidance Molecule A Antibody; Repulsive guidance molecule A; RGM; RGM domain family member A; RGMA; RGMA_HUMAN; anti-RGMA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Repulsive Guidance Molecule A Antibody detects endogenous levels of total Repulsive Guidance Molecule A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 450

• Applicable Applications for anti-RGMA antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Repulsive Guidance Molecule A, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using Repulsive Guidance Molecule A Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Rat Brain, using Repulsive Guidance Molecule A Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612381 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612381 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-RGMA antibody

Function: Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

Post Translational Modifications: Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds.

Subcellular Location: Cell membrane>Lipid-anchor.

Subunit Structure: Interacts with NEO1, BMP2 and BMP4.

Similarity: Belongs to the repulsive guidance molecule (RGM) family.

NCBI and Uniprot Product Information

• NCBI GI #: 296452979
• NCBI GeneID: 56963
• UniProt Accession #: Q96B86
• Molecular Weight: Observed Molecular Weight: (Observed)49kD.; Predicted Molecular Weight: (Calculated)49kDa.
• NCBI Official Full Name: Repulsive guidance molecule A
• NCBI Official Synonym Full Names: repulsive guidance molecule BMP co-receptor a
• NCBI Official Symbol: RGMA
• NCBI Official Synonym Symbols: RGM
• NCBI Protein Information: repulsive guidance molecule A
• UniProt Protein Name: Repulsive guidance molecule A
• Protein Family: Repulsive guidance molecule
• UniProt Gene Name: RGMA
• UniProt Synonym Gene Names: RGM
• UniProt Entry Name: RGMA_HUMAN

NCBI Description

This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]