Western Blot (WB)
(Western blot analysis of extracts from various samples, using PFD6 Antibody.Lane 1: EC304 cells, treated with blocking peptide;Lane 2: EC304 cells;Lane 3: 3T3.)
Rabbit PFD6 Polyclonal Antibody | anti-PFDN6 antibody
PFD6 Antibody
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from various samples, using PFD6 Antibody.Lane 1: EC304 cells, treated with blocking peptide;Lane 2: EC304 cells;Lane 3: 3T3.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using PFD6 Antibody.Lane 1: EC304 cells, treated with blocking peptide;Lane 2: EC304 cells;Lane 3: 3T3.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612281 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612281 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Subunit Structure: Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.
Similarity: Belongs to the prefoldin subunit beta family.
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)15kDa.
NCBI Description
PFDN6 is a subunit of the heteromeric prefoldin complex that chaperones nascent actin (see MIM 102560) and alpha- and beta-tubulin (see MIM 602529 and MIM 191130, respectively) chains pending their transfer to the cytosolic chaperonin containing TCP1 (MIM 186980) (CCT) complex (Hansen et al., 1999 [PubMed 10209023]).[supplied by OMIM, Jul 2010]
Uniprot Description
PFDN6: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit beta family.
Protein type: Motility/polarity/chemotaxis; Chaperone
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: cytoplasm; prefoldin complex
Molecular Function: chaperone binding; unfolded protein binding
Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; chaperone-mediated protein complex assembly
Research Articles on PFDN6
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Product Notes
The PFDN6 pfdn6 (Catalog #AAA9612281) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFD6 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's PFD6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PFDN6 pfdn6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFD6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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