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Immunohistochemistry (IHC)
(At 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Rabbit SKI Polyclonal Antibody | anti-SKI antibody
Phospho-SKI (Ser383) Antibody
Gene Names
SKI; SGS; SKV
Reactivity
Human, Mouse, RatPredicted: Bovine, Horse, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
SKI; Polyclonal Antibody; Phospho-SKI (Ser383) Antibody; Avian sarcoma viral (v ski) oncogene homolog; C oncogene; C ski; Proto-oncogene c-Ski; Ski oncogene; Ski oncoprotein; SKI_HUMAN; SKV; v ski avian sarcoma viral oncogene homolog; v ski sarcoma viral oncogene homolog; anti-SKI antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Horse, Dog, Chicken, Xenopus
Predicted: Bovine, Horse, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-SKI(Ser383) Antibody detects endogenous levels of SKI only when phosphorylated at Ser383.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
728
Applicable Applications for anti-SKI antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human SKI around the phosphorylation site of Ser383.
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(At 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemmistry (IHC)
(At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemmistry (IHC)
(At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Western Blot (WB)
(Western blot analysis of Phospho-SKI(Ser383) in lysates of Jurkat, using Phospho-SKI(Ser383) Antibody.)
Western Blot (WB)
(Western blot analysis of Phospho-SKI(Ser383) in lysates of Jurkat, using Phospho-SKI(Ser383) Antibody.)
Related Product Information for anti-SKI antibody
Description: May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Post Translational Modifications: Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling.
Subunit Structure: Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes.
Similarity: Belongs to the SKI family.
Post Translational Modifications: Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling.
Subunit Structure: Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes.
Similarity: Belongs to the SKI family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 80 kDa
Predicted: 81 kDa
Predicted: 81 kDa
NCBI Official Full Name
Ski oncogene
NCBI Official Synonym Full Names
SKI proto-oncogene
NCBI Official Symbol
SKI
NCBI Official Synonym Symbols
SGS; SKV
NCBI Protein Information
ski oncogene
UniProt Protein Name
Ski oncogene
Protein Family
UniProt Gene Name
SKI
UniProt Entry Name
SKI_HUMAN
NCBI Description
This gene encodes the nuclear protooncogene protein homolog of avian sarcoma viral (v-ski) oncogene. It functions as a repressor of TGF-beta signaling, and may play a role in neural tube development and muscle differentiation. [provided by RefSeq, Oct 2009]
Research Articles on SKI
Similar Products
Product Notes
The SKI ski (Catalog #AAA9610398) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SKI (Ser383) Antibody reacts with Human, Mouse, Rat Predicted: Bovine, Horse, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's SKI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the SKI ski for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SKI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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