Rat Follistatin (FS) ELISA Kit | FS elisa kit

Rat Follistatin (FS) ELISA Kit

Cat. Num. AAA2025570

• Gene Names: FST; FS
• Reactivity: Rat
• Synonyms: Follistatin (FS); Rat Follistatin (FS) ELISA Kit; FST; Activin-binding protein; FS elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Follistatin (FS).; No significant cross-reactivity or interference between Follistatin (FS) and analogues was observed.
• Sequence Length: 317

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 1.23-100ng/mL
• Sensitivity: < 0.52ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin (FS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin (FS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FS elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Follistatin (FS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Follistatin (FS) and unlabeled Follistatin (FS) (Standards or samples) with the pre-coated antibody specific to Follistatin (FS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Follistatin (FS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Follistatin (FS) in the sample.

Product Categories/Family for FS elisa kit

Endocrinology; Reproductive science; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 182721
• NCBI GeneID: 10468
• Molecular Weight: 34,803 Da
• NCBI Official Full Name: follistatin
• NCBI Official Synonym Full Names: follistatin
• NCBI Official Symbol: FST
• NCBI Official Synonym Symbols: FS
• NCBI Protein Information: follistatin
• UniProt Protein Name: Follistatin
• Protein Family: Follistatin
• UniProt Gene Name: FST
• UniProt Synonym Gene Names: FS
• UniProt Entry Name: FST_HUMAN

NCBI Description

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. [provided by RefSeq, Jul 2008]

Uniprot Description

FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: heparan sulfate proteoglycan binding; signal transducer activity; protein binding; activin binding

Biological Process: hair follicle morphogenesis; negative regulation of transcription from RNA polymerase II promoter; keratinocyte proliferation; pattern specification process; odontogenesis of dentine-containing teeth; BMP signaling pathway; positive regulation of hair follicle development; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; gamete generation; hemopoietic progenitor cell differentiation; skeletal development; female gonad development

Disease: Polycystic Ovary Syndrome 1