Western Blot (WB)
(Western blot analysis of Ski in HeLa cell lysate with Ski antibody at (A) 1 and (B) 2 μg/mL. )
Rabbit anti-Human Ski Polyclonal Antibody | anti-SKI antibody
Ski Antibody
Gene Names
SKI; SGS; SKV
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Ski Antibody is affinity chromatography purified via peptide column.
Synonyms
Ski; Polyclonal Antibody; Ski Antibody; SGS; SKV; Ski oncogene; Proto-oncogene c-Ski; v-ski sarcoma viral oncogene homolog (avian); anti-SKI antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Ski Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Sequence Length
728
Applicable Applications for anti-SKI antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
SkiP antibody can be used for detection of Ski by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
Ski antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Ski.
Buffer
Ski Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Ski antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of Ski in HeLa cell lysate with Ski antibody at (A) 1 and (B) 2 μg/mL. )
Western Blot (WB)
(Western blot analysis of Ski in HeLa cell lysate with Ski antibody at (A) 1 and (B) 2 μg/mL. )
Immunofluorescence (IF)
(Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of Ski in Human Kidney cells with Ski antibody at 20 μg/mL.)
Related Product Information for anti-SKI antibody
Ski Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
80,005 Da
NCBI Official Full Name
Ski oncogene
NCBI Official Synonym Full Names
SKI proto-oncogene
NCBI Official Symbol
SKI
NCBI Official Synonym Symbols
SGS; SKV
NCBI Protein Information
ski oncogene; ski oncoprotein; proto-oncogene c-Ski; v-ski avian sarcoma viral oncogene homolog
UniProt Protein Name
Ski oncogene
Protein Family
UniProt Gene Name
SKI
UniProt Entry Name
SKI_HUMAN
NCBI Description
This gene encodes the nuclear protooncogene protein homolog of avian sarcoma viral (v-ski) oncogene. It functions as a repressor of TGF-beta signaling, and may play a role in neural tube development and muscle differentiation. [provided by RefSeq, Oct 2009]
Uniprot Description
May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Research Articles on SKI
Similar Products
Product Notes
The SKI ski (Catalog #AAA150393) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ski Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ski can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). SkiP antibody can be used for detection of Ski by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the SKI ski for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ski, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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