Western Blot (WB)
(Western blot analysis of TOP1MT using COLO205 whole cell lysates)
Rabbit TOP1MT Polyclonal Antibody | anti-TOP1MT antibody
TOP1MT Antibody
ELISA: 1:20000-1:40000
The optimal dilutions should be determined by the end user.
Important Note: For western blots , incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night.
Western Blot (WB)
(Western blot analysis of TOP1MT using COLO205 whole cell lysates)
Western Blot (WB)
(Western blot analysis of TOP1MT using COLO205 whole cell lysates)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Uniprot Description
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone ().
Research Articles on TOP1MT
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Product Notes
The TOP1MT top1mt (Catalog #AAA9609769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOP1MT Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TOP1MT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000 ELISA: 1:20000-1:40000 The optimal dilutions should be determined by the end user. Important Note: For western blots, incubate membrance with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking over night. Researchers should empirically determine the suitability of the TOP1MT top1mt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOP1MT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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