anti-Human ATG4D Antibody | anti-ATG4D antibody

ATG4D Antibody

Cat. Num. AAA858095

• Gene Names: ATG4D; APG4D; AUTL4; APG4-D
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ATG4D; ATG4D Antibody; APG4 autophagy 4 homolog D; APG4-D; APG4D; ATG4 autophagy related 4 homolog D (S. cerevisiae); AUT-like 4 cysteine endopeptidase; AUT-like 4; cysteine endopeptidase; AUTL4; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D; Cysteine protease ATG4D; cysteine protease involved in autophagy; anti-ATG4D antibody

• Reactivity: Human
• Specificity: ATG4D antibody detects endogenous levels of total ATG4Dprotein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 411

• Applicable Applications for anti-ATG4D antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from N-terminal of human ATG4D.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

Product Categories/Family for anti-ATG4D antibody

Autophagy antibody

References

Marino G., J. Biol. Chem. 278:3671-3678(2003). The MGC Project Team, Genome Res. 14:2121-2127(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 528078306
• NCBI GeneID: 84971
• NCBI Accession #: NP_001268433.1
• NCBI GenBank Nucleotide #: NM_001281504.1
• UniProt Accession #: Q86TL0; Q969K0
• Molecular Weight: 16,008 Da
• NCBI Official Full Name: cysteine protease ATG4D isoform 2
• NCBI Official Synonym Full Names: autophagy related 4D cysteine peptidase
• NCBI Official Symbol: ATG4D
• NCBI Official Synonym Symbols: APG4D; AUTL4; APG4-D
• NCBI Protein Information: cysteine protease ATG4D
• UniProt Protein Name: Cysteine protease ATG4D
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4D
• UniProt Synonym Gene Names: APG4D; AUTL4

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.