Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using GBP1 antibody.The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human GBP1 Polyclonal Antibody | anti-GBP1 antibody
GBP1 Antibody
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GBP1; Polyclonal Antibody; GBP1 Antibody; GBP 1; GBP-1; GBP1_HUMAN; GTP binding protein 1; GTP-binding protein 1; Guanine nucleotide binding protein 1; Guanine nucleotide-binding protein 1; Guanylate binding protein 1; Guanylate binding protein 1 interferon inducible 67kDa; Guanylate binding protein 1 interferon inducible; HuGBP 1; HuGBP-1; HuGBP1; Interferon induced guanylate binding protein 1; Interferon-induced guanylate-binding protein 1; OTTHUMP00000012352; anti-GBP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
GBP1 antibody detects endogenous levels of total GBP1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
592
Applicable Applications for anti-GBP1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
By IFNG during macrophage activation, and by TNF and IL1B.
Predicted Cross Reactivity
Horse, Rabbit
Similarity
Horse (83%), Rabbit (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using GBP1 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using GBP1 antibody.The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescene (IF)
(MBS9605054 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9605054 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-GBP1 antibody
Function: Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.
Subunit Structure: Homodimer; homodimerization occurs upon GTP-binding and is required for the second hydrolysis step from GDP to GMP. Heterodimer with other family members, including GBP2, GBP3, GBP4 and GBP5. Dimerization regulates subcellular location to membranous structures.
Post-translational Modifications: Isoprenylation is required for proper subcellular location.
Similarity: Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.
Subunit Structure: Homodimer; homodimerization occurs upon GTP-binding and is required for the second hydrolysis step from GDP to GMP. Heterodimer with other family members, including GBP2, GBP3, GBP4 and GBP5. Dimerization regulates subcellular location to membranous structures.
Post-translational Modifications: Isoprenylation is required for proper subcellular location.
Similarity: Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 68 kD
Predicted: 68 kDa
Predicted: 68 kDa
NCBI Official Full Name
guanylate-binding protein 1
NCBI Official Synonym Full Names
guanylate binding protein 1
NCBI Official Symbol
GBP1
NCBI Protein Information
guanylate-binding protein 1
UniProt Protein Name
Guanylate-binding protein 1
Protein Family
UniProt Gene Name
GBP1
UniProt Synonym Gene Names
GBP-1; HuGBP-1
NCBI Description
Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. [provided by RefSeq, Jul 2008]
Uniprot Description
Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.
Research Articles on GBP1
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Product Notes
The GBP1 gbp1 (Catalog #AAA9605054) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GBP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GBP1 gbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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