Rabbit anti-Human Interferon-induced guanylate-binding protein 1 Polyclonal Antibody | anti-GBP1 antibody

Rabbit anti-human Interferon-induced guanylate-binding protein 1 polyclonal Antibody(GBP1)

Cat. Num. AAA1499104

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: Interferon-induced guanylate-binding protein 1; Polyclonal Antibody; Rabbit anti-human Interferon-induced guanylate-binding protein 1 polyclonal Antibody(GBP1); GTP-binding protein 1; GBP-1; HuGBP-1; Guanine nucleotide-binding protein 1; GBP1; anti-GBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 592

• Applicable Applications for anti-GBP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Interferon-induced guanylate-binding protein 1 protein (383-592aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10579 / sc-28579
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Interferon-induced guanylate-binding protein 1 antibody at 4ug/mlLane 1:MM231 whole cell lysateLane 2:Hela whole cell lysateLane 3:MGF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 68kDaObserved band size: 68kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil using MBS1499104 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human rectum using MBS1499104 at dilution 1:100)

Immunoprecipitation (IP)

(Immunoprecipitating GBP1 in MCF-7 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1499104 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1499104(4ug)+ MCF-7 whole cell lysate(500ug)Lane 3: MCF-7 whole cell lysate (20ug))

Related Product Information for anti-GBP1 antibody

Hydrolyzes GTP to GMP in two consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.

NCBI and Uniprot Product Information

• NCBI GI #: 166706903
• NCBI GeneID: 2633
• NCBI Accession #: NP_002044.2
• NCBI GenBank Nucleotide #: NM_002053.2
• UniProt Accession #: P32455; Q5T8M1; D3DT26
• Molecular Weight: 67,931 Da
• NCBI Official Full Name: guanylate-binding protein 1
• NCBI Official Synonym Full Names: guanylate binding protein 1
• NCBI Official Symbol: GBP1
• NCBI Protein Information: guanylate-binding protein 1
• UniProt Protein Name: Guanylate-binding protein 1
• Protein Family: Guanylate-binding protein
• UniProt Gene Name: GBP1
• UniProt Synonym Gene Names: GBP-1; HuGBP-1
• UniProt Entry Name: GBP1_HUMAN

NCBI Description

Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. [provided by RefSeq, Jul 2008]

Uniprot Description

GBP1: a member of the family of large GTP-binding proteins (GBPs) that is inducible by interferon. Possesses a high GTP hydrolysis activity. GBPs are the most abundant cellular proteins expressed in response to interferon-gamma (IFN-gamma). IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) strongly induce the expression of GBP-1, -2, and -3.

Protein type: Vesicle; Hydrolase; Cell adhesion

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: cytosol; extracellular region; Golgi membrane; plasma membrane

Molecular Function: actin binding; cytokine binding; enzyme binding; GTP binding; GTPase activity; Hsp90 protein binding; identical protein binding; protein binding; spectrin binding

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; metabolic process; negative regulation of T cell receptor signaling pathway; regulation of calcium-mediated signaling