Rabbit anti-Human GBP1 Polyclonal Antibody | anti-GBP1 antibody

GBP1, NT (GBP1, Interferon-induced guanylate-binding protein 1, GTP-binding protein 1, Guanine nucleotide-binding protein 1) (AP)

Cat. Num. AAA6301456

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: GBP1; Polyclonal Antibody; NT (GBP1; Interferon-induced guanylate-binding protein 1; GTP-binding protein 1; Guanine nucleotide-binding protein 1) (AP); Guanine nucleotide-binding protein 1; anti-GBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GBP1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-GBP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 141-170 in the N-terminal region of human GBP1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS6005684. This demonstrates that MBS6005684 detected the GBP1 protein (arrow).)

Related Product Information for anti-GBP1 antibody

Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3.

Product Categories/Family for anti-GBP1 antibody

Antibodies; Binding Proteins

References

1. Vopel, T., et al. J. Mol. Biol. 400(1):63-70(2010). 2. Mirpuri, J., et al. J. Immunol. 184(12):7186-7195(2010). 3. Lipnik, K., et al. Mol. Med. 16 (5-6), 177-187 (2010). 4. Davila, S., et al. Genes Immun. 11(3):232-238(2010). 5. O'Doherty, C., et al. Pharmacogenomics 10(7):1177-1186(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 166706903
• NCBI GeneID: 2633
• NCBI Accession #: NP_002044.2
• NCBI GenBank Nucleotide #: NM_002053.2
• UniProt Accession #: P32455; Q5T8M1; D3DT26
• Molecular Weight: 67,931 Da
• NCBI Official Full Name: interferon-induced guanylate-binding protein 1
• NCBI Official Synonym Full Names: guanylate binding protein 1, interferon-inducible
• NCBI Official Symbol: GBP1
• NCBI Protein Information: interferon-induced guanylate-binding protein 1; GBP-1; huGBP-1; GTP-binding protein 1; guanine nucleotide-binding protein 1; guanylate binding protein 1, interferon-inducible, 67kDa
• UniProt Protein Name: Interferon-induced guanylate-binding protein 1
• Protein Family: Guanylate-binding protein
• UniProt Gene Name: GBP1
• UniProt Synonym Gene Names: GBP-1; HuGBP-1
• UniProt Entry Name: GBP1_HUMAN

NCBI Description

Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. [provided by RefSeq, Jul 2008]

Uniprot Description

GBP1: a member of the family of large GTP-binding proteins (GBPs) that is inducible by interferon. Possesses a high GTP hydrolysis activity. GBPs are the most abundant cellular proteins expressed in response to interferon-gamma (IFN-gamma). IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) strongly induce the expression of GBP-1, -2, and -3.

Protein type: Vesicle; Cell adhesion; Hydrolase

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: Golgi membrane; extracellular region; cytosol

Molecular Function: GTPase activity; identical protein binding; GTP binding

Biological Process: metabolic process; cytokine and chemokine mediated signaling pathway; defense response to virus