Immunohistochemistry (IHC)
(MBS9603624 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit ETV5 Polyclonal Antibody | anti-ETV5 antibody
ETV5 Antibody
Gene Names
ETV5; ERM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Synonyms
ETV5; Polyclonal Antibody; ETV5 Antibody; ERM; Ets related protein ERM; ETS translocation variant 5; Ets variant gene 5; Ets-related protein ERM; ETV5_HUMAN; anti-ETV5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ERM/ETV5 Ab detects endogenous levels of total ERM/ETV5.
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
510
Applicable Applications for anti-ETV5 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA: 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
ELISA: 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human ERM/ETV5, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603624 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603624 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts from B16F10, using ETV5 antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from B16F10, using ETV5 antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts of PC-3 cells, using ETV5 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of PC-3 cells, using ETV5 antibody.)
Related Product Information for anti-ETV5 antibody
Function: Binds to DNA sequences containing the consensus nucleotide core sequence 5'-GGAA.-3'.
Subunit Structure: Interacts (via C-terminal) with ZMYM5 (via N-terminal 120 amino acid region).
Similarity: Belongs to the ETS family.
Subunit Structure: Interacts (via C-terminal) with ZMYM5 (via N-terminal 120 amino acid region).
Similarity: Belongs to the ETS family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58 kDa
NCBI Official Full Name
ETS translocation variant 5
NCBI Official Synonym Full Names
ETS variant 5
NCBI Official Symbol
ETV5
NCBI Official Synonym Symbols
ERM
NCBI Protein Information
ETS translocation variant 5
UniProt Protein Name
ETS translocation variant 5
Protein Family
UniProt Gene Name
ETV5
UniProt Synonym Gene Names
ERM
Uniprot Description
Binds to DNA sequences containing the consensus nucleotide core sequence 5'-GGAA.-3'.
Research Articles on ETV5
Similar Products
Product Notes
The ETV5 etv5 (Catalog #AAA9603624) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ETV5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ETV5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA: 1:20000-1:40000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the ETV5 etv5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ETV5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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