CCAAT/Enhancer Binding Protein Gamma (CEBPg) Recombinant Protein | CEBPg recombinant protein

Recombinant CCAAT/Enhancer Binding Protein Gamma (CEBPg)

Cat. Num. AAA2018727

• Gene Names: CEBPG; GPE1BP; IG/EBP-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: CCAAT/Enhancer Binding Protein Gamma (CEBPg); Recombinant CCAAT/Enhancer Binding Protein Gamma (CEBPg); CEBPg recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: Residues: Met1~Gln150 (Accession # P53567), with two N-terminal Tags, His-tag and T7-tag. The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSKISQQNST PGVNGISVIH TQAHASGLQQ VPQLVPAGPG GGGKAVAPSK QSKKSSPMDR NSDEYRQRRE RNNMAVKKSR LKSKQKAQDT LQRVNQLKEE NERLEAKIKL LTKELSVLKD LFLEHAHNLA DNVQSISTEN TTADGDNAGQ
• Sequence Length: 150

• Applicable Applications for CEBPg recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 356582519
• NCBI GeneID: 1054
• NCBI Accession #: NP_001239225.1
• NCBI GenBank Nucleotide #: NM_001252296.1
• UniProt Accession #: P53567; Q5U052; B2R946
• Molecular Weight: 20.2kDa
• NCBI Official Full Name: CCAAT/enhancer-binding protein gamma
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), gamma
• NCBI Official Symbol: CEBPG
• NCBI Official Synonym Symbols: GPE1BP; IG/EBP-1
• NCBI Protein Information: CCAAT/enhancer-binding protein gamma; c/EBP gamma
• UniProt Protein Name: CCAAT/enhancer-binding protein gamma
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: CEBPG
• UniProt Synonym Gene Names: C/EBP gamma
• UniProt Entry Name: CEBPG_HUMAN

NCBI Description

The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

C/EBP-gamma: Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites. Belongs to the bZIP family. C/EBP subfamily.

Protein type: Transcription factor; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; transcription factor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; positive regulation of DNA repair; transcription, DNA-dependent; natural killer cell mediated cytotoxicity; negative regulation of transcription factor activity; B cell differentiation; immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; enucleate erythrocyte differentiation; liver development; mRNA metabolic process; positive regulation of DNA binding; positive regulation of interferon-gamma biosynthetic process