Rabbit RNF156 Polyclonal Antibody | anti-RNF156 antibody

RNF156 Antibody

Cat. Num. AAA9603540

• Gene Names: MGRN1; RNF156
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RNF156; Polyclonal Antibody; RNF156 Antibody; E3 ubiquitin-protein ligase MGRN1; KIAA0544; Mahogunin ring finger 1; Mahogunin RING finger protein 1; MGRN 1; Mgrn1; MGRN1_HUMAN; Probable E3 ubiquitin protein ligase MGRN1; RING finger protein 156; RNF 156; anti-RNF156 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RNF156 antibody detects endogenous levels of RNF156.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 554

• Applicable Applications for anti-RNF156 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human RNF156.
• Subcellular Location: Cytoplasm > Cytosol. Cell Membrane; Early Endosome. The endosomal localization is dependent on the interaction with TSG101 and Cytoplasm > Cytosol. Nucleus. Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (92%), Bovine (92%), Horse (92%), Rabbit (92%), Dog (92%), Chicken (100%), Xenopus (92%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of HepG2 cells, using RNF156 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from various samples, using RNF156 antibody. Lane 1: mouse brain treated with blocking peptide. Lane 2: Mouse brain; Lane 3: 3T3; Lane 4: Hela; )

Immunohistochemistry (IHC)

(MBS9603540 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-RNF156 antibody

Function: E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R-and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling (By similarity).
Subunit Structure: Interacts with MC1R and MC4R, but not with TBXA2R. Interacts with TSG101. Interacts with mislocalized cytosolically exposed PRNP; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement (By similarity).
Post-translational Modifications: Autoubiquitinated in vitro.
Similarity: The RING finger is required for ubiquitin ligase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 334883178
• NCBI GeneID: 23295
• NCBI Accession #: NP_001135761.2
• NCBI GenBank Nucleotide #: NM_001142289.2
• UniProt Accession #: O60291; Q86W76; A4URL3; A4URL4
• Molecular Weight: Observed: 66-72 kDa; 55 kDa; Predicted: 61 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase MGRN1 isoform 2
• NCBI Official Synonym Full Names: mahogunin ring finger 1
• NCBI Official Symbol: MGRN1
• NCBI Official Synonym Symbols: RNF156
• NCBI Protein Information: E3 ubiquitin-protein ligase MGRN1
• UniProt Protein Name: E3 ubiquitin-protein ligase MGRN1
• UniProt Gene Name: MGRN1
• UniProt Synonym Gene Names: KIAA0544; RNF156

NCBI Description

Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.[supplied by OMIM, Apr 2004]

Uniprot Description

E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling ().