Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-231 cells, using PPIG Antibody. The lane on the left was treated with blocking peptide.)
Rabbit PPIG Polyclonal Antibody | anti-PPIG antibody
PPIG Antibody
Gene Names
PPIG; CYP; SRCyp; SCAF10; CARS-Cyp
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
PPIG; Polyclonal Antibody; PPIG Antibody; CARS cyclophilin; CARS Cyp; CARS-cyclophilin; CARS-Cyp; CASP 10; CASP10; Clk associating RS cyclophilin; Clk-associating RS-cyclophilin; Cyclophilin G; CyclophilinG; CYP; MGC133241; Peptidyl prolyl cis trans isomerase G; Peptidyl prolyl isomerase G (cyclophilin G); Peptidyl prolyl isomerase G; Peptidyl-prolyl cis-trans isomerase G; Peptidyl-prolyl isomerase G; Peptidylprolyl isomerase G (cyclophilin G); Peptidylprolyl isomerase G; PPIase G; Ppig; PPIG_HUMAN; Rotamase G; RotamaseG; SCAF10; SR cyclophilin; SR cyp; SR related CTD associated factor 10; SR-cyclophilin; SR-cyp; SRcyp; anti-PPIG antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
PPIG Antibody detects endogenous levels of total PPIG.
Tissue Specificity: Ubiquitous.
Tissue Specificity: Ubiquitous.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PPIG antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human PPIG, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Interacts with CLK1, PNN and with the phosphorylated C-terminal domain of RNA polymerase II.
Similarity
The RS domain is required for the interaction with the phosphorylated C-terminal domain of RNA polymerase II.
Subcellular Location
Nucleus matrix. Nucleus speckle.
Note: Colocalizes with RNA splicing factors at nuclear speckles.
Note: Colocalizes with RNA splicing factors at nuclear speckles.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-231 cells, using PPIG Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-231 cells, using PPIG Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293 , using PPIG Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293 , using PPIG Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-PPIG antibody
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
754
NCBI Official Full Name
Peptidyl-prolyl cis-trans isomerase G
NCBI Official Synonym Full Names
peptidylprolyl isomerase G (cyclophilin G)
NCBI Official Symbol
PPIG
NCBI Official Synonym Symbols
CYP; SRCyp; SCAF10; CARS-Cyp
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase G; CASP10; SR-cyp; PPIase G; rotamase G; cyclophilin G; SR-cyclophilin; CARS-cyclophilin; Clk-associating RS-cyclophilin; SR-related CTD-associated factor 10; peptidyl-prolyl isomerase G (cyclophilin G)
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase G
Protein Family
UniProt Gene Name
PPIG
UniProt Synonym Gene Names
PPIase G; Peptidyl-prolyl isomerase G; CARS-Cyp; CARS-cyclophilin; SR-cyclophilin; SR-cyp; SRcyp
UniProt Entry Name
PPIG_HUMAN
Uniprot Description
PPIG: an ubiquitous peptidyl-prolyl cis/trans isomerase. Distributed in nuclear speckles, which are rich in splicing factors. Colocalizes with the splicing factor SFRS2. A component of splicing factor complexes that bind the phosphorylated C-terminal domain (CTD) of RNA polymerase II. Interacts with CLK1 and PNN. Two splice variant isoforms of the human protein have been described.
Protein type: Isomerase; EC 5.2.1.8; Cyclophilin
Chromosomal Location of Human Ortholog: 2q31.1
Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nuclear speck; nucleus
Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding
Biological Process: protein peptidyl-prolyl isomerization; protein folding; RNA splicing
Research Articles on PPIG
Similar Products
Product Notes
The PPIG ppig (Catalog #AAA9613473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPIG Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%) and may cross-react with other species as described in the data sheet. AAA Biotech's PPIG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PPIG ppig for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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