Rabbit PRKCDBP Polyclonal Antibody | anti-PRKCDBP antibody

PRKCDBP Antibody

Cat. Num. AAA9603263

• Gene Names: CAVIN3; SRBC; HSRBC; PRKCDBP; cavin-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: PRKCDBP; Polyclonal Antibody; PRKCDBP Antibody; Cavin 3; Cavin-3; CAVIN3; hSRBC; MGC20400; PRDBP_HUMAN; Prkcdbp; Protein kinase C delta binding protein; Protein kinase C delta-binding protein; Sdr related gene product that binds to c kinase; Serum deprivation response factor related gene product that binds to C kinase; Serum deprivation response factor-related gene product that binds to C-kinase; SRBC; anti-PRKCDBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PRKCDBP antibody detects endogenous levels of PRKCDBP.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-PRKCDBP antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human PRKCDBP.
• Subcellular Location: Cytoplasm.
• Tissue Specificity: Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Strongly expressed in mammary and epithelial cells.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Pig (100%), Bovine (91%), Horse (100%), Sheep (82%), Rabbit (91%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603263 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of MCF-7 cells, using PRKCDBP antibody.)

Related Product Information for anti-PRKCDBP antibody

Function: Regulates the traffic and/or budding of caveolae (PubMed:19262564). Plays a role in caveola formation in a tissue-specific manner. Required for the formation of caveolae in smooth muscle but not in the lung and heart endothelial cells. Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by promoting the rapid release of caveolae from the cell surface. Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2 (By similarity).
Subunit Structure: Component of the CAVIN complex composed of CAVIN1, CAVIN2, CAVIN3 and CAVIN4. Interacts with PRKCD and with phosphatidylserine. Phosphatidylserine may form a bridge between PKC and PKC-binding partners and stabilize the binding. Interacts with PER2. Interacts with CAVIN1 (By similarity). Interacts (via leucine-zipper domain) with CAV1 in a cholesterol-sensitive manner (PubMed:19262564). Interacts with EPS15L1 (PubMed:19262564).
Post-translational Modifications: In vitro, phosphorylated by PRKCD.
Similarity: The leucine-zipper domain is essential for its localization in the caveolae and for its interaction with CAV1 and EPS15L1. Belongs to the CAVIN family.

NCBI and Uniprot Product Information

• NCBI GI #: 47132587
• NCBI GeneID: 112464
• NCBI Accession #: NP_659477.2
• NCBI GenBank Nucleotide #: NM_145040.2
• UniProt Accession #: Q969G5
• Molecular Weight: Observed: 35-40 kDa; Predicted: 28 kDa
• NCBI Official Full Name: caveolae-associated protein 3
• NCBI Official Synonym Full Names: caveolae associated protein 3
• NCBI Official Symbol: CAVIN3
• NCBI Official Synonym Symbols: SRBC; HSRBC; PRKCDBP; cavin-3
• NCBI Protein Information: caveolae-associated protein 3
• UniProt Protein Name: Caveolae-associated protein 3
• Protein Family: Protein kinase C delta-binding protein
• UniProt Gene Name: CAVIN3
• UniProt Synonym Gene Names: PRKCDBP; SRBC; hSRBC

NCBI Description

The protein encoded by this gene was identified as a binding protein of the protein kinase C, delta (PRKCD). The expression of this gene in cultured cell lines is strongly induced by serum starvation. The expression of this protein was found to be down-regulated in various cancer cell lines, suggesting the possible tumor suppressor function of this protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Regulates the traffic and/or budding of caveolae (PubMed:19262564). Plays a role in caveola formation in a tissue-specific manner. Required for the formation of caveolae in smooth muscle but not in the lung and heart endothelial cells. Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by promoting the rapid release of caveolae from the cell surface. Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2 ().