Western Blot (WB)
(Western blot analysis of lysate from A549 cells, using GCSc-gamma antibody.)
Rabbit GCSc-gamma Polyclonal Antibody | anti-GCSc-gamma antibody
GCSc-gamma Antibody
Gene Names
GCLC; GCL; GCS; GLCL; GLCLC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
GCSc-gamma; Polyclonal Antibody; GCSc-gamma Antibody; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCLC; GLCL; GLCLC; anti-GCSc-gamma antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
GCSc-gamma antibody detects endogenous levels of GCSc-gamma.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
637
Applicable Applications for anti-GCSc-gamma antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:10000
ELISA: 1:10000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human GCSc-gamma.
Immunogen Range
266-315
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysate from A549 cells, using GCSc-gamma antibody.)
Western Blot (WB)
(Western blot analysis of lysate from A549 cells, using GCSc-gamma antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72 kDa
NCBI Official Full Name
glutamate--cysteine ligase catalytic subunit isoform b
NCBI Official Synonym Full Names
glutamate-cysteine ligase catalytic subunit
NCBI Official Symbol
GCLC
NCBI Official Synonym Symbols
GCL; GCS; GLCL; GLCLC
NCBI Protein Information
glutamate--cysteine ligase catalytic subunit
UniProt Protein Name
Glutamate--cysteine ligase catalytic subunit
UniProt Gene Name
GCLC
UniProt Synonym Gene Names
GLCL; GLCLC
NCBI Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
Research Articles on GCSc-gamma
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Product Notes
The GCSc-gamma gclc (Catalog #AAA9508847) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GCSc-gamma Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GCSc-gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the GCSc-gamma gclc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCSc-gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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