SDS-Page
Interleukin 17D Recombinant Protein | IL17D recombinant protein
Recombinant Interleukin 17D (IL17D)
Gene Names
IL17D; IL27; IL-27; IL-17D
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Interleukin 17D; Recombinant Interleukin 17D (IL17D); Interleukin-27; IL17D recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Sequence
YVVKQ KIPGTLGDEG RGQRLVFVNC SSMVTAGATD QVCVAAFWLQ PPLPPCVAKP HTSSSRSLQH SPDPPLELAM IPMPEARGLA CPQACVILEA GPTSLPSSPG ISYDPARFPR YLPEAYCLCR GCLTGLYGEE DFRFRSAPVF SPAVVLRRTA ACAGGRSVYA EHYITIPVGCTCVPEPDKSA DSANSSMDKM LLGV
Sequence Length
202
Applicable Applications for IL17D recombinant protein
SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
Predicted Molecular Mass
24.9kDa
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Endotoxin Level
<1.0EU per 1 ug (determined by the LAL method)
Expression System
Prokaryotic expression
Fragment
Tyr76~Val274
Organism Species
Rat
Tag
two N-terminal Tags, His-tag and T7-tag
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,893 Da
NCBI Official Full Name
interleukin-17D
NCBI Official Synonym Full Names
interleukin 17D
NCBI Official Symbol
IL17D
NCBI Official Synonym Symbols
IL27; IL-27; IL-17D
NCBI Protein Information
interleukin-17D
UniProt Protein Name
Interleukin-17D
Protein Family
UniProt Gene Name
IL17D
UniProt Synonym Gene Names
IL27; IL-17D; IL-27
UniProt Entry Name
IL17D_HUMAN
NCBI Description
The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent. [provided by RefSeq, Jul 2008]
Uniprot Description
IL17D: Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells. Belongs to the IL-17 family.
Protein type: Cytokine; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 13q11
Cellular Component: extracellular space
Molecular Function: cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding
Biological Process: cell surface receptor linked signal transduction; inflammatory response
Research Articles on IL17D
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Product Notes
The IL17D il17d (Catalog #AAA2033502) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interleukin 17D can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the IL17D il17d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: YVVKQ KIPGTLGDEG RGQRLVFVNC SSMVTAGATD QVCVAAFWLQ PPLPPCVAKP HTSSSRSLQH SPDPPLELAM IPMPEARGLA CPQACVILEA GPTSLPSSPG ISYDPARFPR YLPEAYCLCR GCLTGLYGEE DFRFRSAPVF SPAVVLRRTA ACAGGRSVYA EHYITIPVGC TCVPEPDKSA DSANSSMDKM LLGV. It is sometimes possible for the material contained within the vial of "Interleukin 17D, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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