Human antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 ELISA Kit | MFI2 elisa kit

Human Melanotransferrin, MFI2 ELISA Kit

Cat. Num. AAA946083

• Gene Names: MFI2; MTF1; CD228; MAP97
• Reactivity: Human
• Synonyms: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5; Human Melanotransferrin; MFI2 ELISA Kit; Human Melanotransferrin (MFI2) ELISA kit; CD228; FLJ38863; MAP97; MGC4856; MTF1; melanoma-associated antigen p97; isoform 2; melanotransferrin; membrane-bound transferrin-like protein; MFI2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MFI2. No significant cross-reactivity or interference between Human MFI2 and analogues was observed.
• Sequence Length: 738

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Transport
• Protein Biological Process 2: Anion transport
• Protein Biological Process 3: Ion transport
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MFI2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for melanotransferrin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any melanotransferrin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for melanotransferrin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of melanotransferrin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 134244281
• NCBI GeneID: 4241
• NCBI Accession #: NP_005920.2
• NCBI GenBank Nucleotide #: NM_005929.5
• UniProt Accession #: P08582; Q9BQE2
• Molecular Weight: 80,215 Da
• NCBI Official Full Name: melanotransferrin isoform 1
• NCBI Official Synonym Full Names: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
• NCBI Official Symbol: MFI2
• NCBI Official Synonym Symbols: MTF1; CD228; MAP97
• NCBI Protein Information: melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2
• UniProt Protein Name: Melanotransferrin
• UniProt Gene Name: MFI2
• UniProt Synonym Gene Names: MAP97
• UniProt Entry Name: TRFM_HUMAN

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: anchored to plasma membrane; cell surface; integral to plasma membrane

Molecular Function: protein binding; ferric iron binding; iron ion binding

Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation