Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serum amyloid A (SAA) Standard Curve.)
Human Serum Amyloid A (SAA) ELISA Kit | SAA elisa kit
Human Serum Amyloid A (SAA) ELISA Kit
Gene Names
Saa2; Saa1; Saa-2; AW111173
Reactivity
Human
Synonyms
Serum Amyloid A (SAA); Human Serum Amyloid A (SAA) ELISA Kit; SAA2; SAA elisa kit
Reactivity
Human
Specificity
Human Serum amyloid A (SAA) ELISA Kit has high sensitivity and excellent specificity for detection of Human SAA2. No significant cross-reactivity or interference between Human SAA2 and analogues was observed.
Sequence Length
122
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serum amyloid A (SAA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serum amyloid A (SAA) Standard Curve.)
Related Product Information for SAA elisa kit
Description: This Human Serum amyloid A (SAA) ELISA Kit employs a two-site sandwich ELISA to quantitate SAA2 in samples. An antibody specific for SAA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SAA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SAA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SAA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Serum amyloid A protein is a protein encoded by the SAA1 gene.The serum amyloid A (SAA) protein is an acute phase apolipoprotein reactant produced mainly by hepatocytes and under regulation of inflammatory cytokines (summarized by Lundmark et al., 2002). The SAA cleavage product, designated amyloid protein A (AA), is deposited systemically as amyloid in vital organs including the liver, spleen, and kidneys in patients with chronic inflammatory diseases. There is very limited structural information about SAA because of its inherent poor solubility in the apolipoprotein form. It is intriguing to understand how such a small protein is able to mediate or directly carry out such a wide range of functions related to inflammatory reaction and other hostdefense mechanisms.
Background: Serum amyloid A protein is a protein encoded by the SAA1 gene.The serum amyloid A (SAA) protein is an acute phase apolipoprotein reactant produced mainly by hepatocytes and under regulation of inflammatory cytokines (summarized by Lundmark et al., 2002). The SAA cleavage product, designated amyloid protein A (AA), is deposited systemically as amyloid in vital organs including the liver, spleen, and kidneys in patients with chronic inflammatory diseases. There is very limited structural information about SAA because of its inherent poor solubility in the apolipoprotein form. It is intriguing to understand how such a small protein is able to mediate or directly carry out such a wide range of functions related to inflammatory reaction and other hostdefense mechanisms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serum amyloid A-2 protein preproprotein
NCBI Official Synonym Full Names
serum amyloid A 2
NCBI Official Symbol
Saa2
NCBI Official Synonym Symbols
Saa1; Saa-2; AW111173
NCBI Protein Information
serum amyloid A-2 protein
UniProt Protein Name
Serum amyloid A-2 protein
UniProt Gene Name
Saa2
Uniprot Description
Major acute phase reactant. Apolipoprotein of the HDL complex.
Research Articles on SAA
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Product Notes
The Human SAA saa2 (Catalog #AAA9714419) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714419 ELISA Kit recognizes Human SAA. It is sometimes possible for the material contained within the vial of "Serum Amyloid A (SAA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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