Rabbit PATZ1 Polyclonal Antibody | anti-PATZ1 antibody

PATZ1 Polyclonal Antibody

Cat. Num. AAA9129054

• Gene Names: PATZ1; ZSG; MAZR; PATZ; RIAZ; ZBTB19; ZNF278; dJ400N23
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: PATZ1; Polyclonal Antibody; PATZ1 Polyclonal Antibody ; MAZR; PATZ; RIAZ; ZBTB19; ZNF278; ZSG;dJ400N23; POZ/BTB and AT hook containing zincfinger 1; anti-PATZ1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 687

• Applicable Applications for anti-PATZ1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 - 1:4000
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-350 of human PATZ1(NP_114440.1).
• Calculated MW: 57kDa/69kDa/74kDa
• Observed MW: 74kDa
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PATZ1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

Related Product Information for anti-PATZ1 antibody

The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q then the hybrid thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein . This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene.

Product Categories/Family for anti-PATZ1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 14670360
• NCBI GeneID: 23598
• NCBI Accession #: NP_055138.2
• NCBI GenBank Nucleotide #: NM_014323.2
• UniProt Accession #: Q9HBE1; Q9HBE2; Q9HBE3; Q9P1A9; Q9UDU0; Q9Y529
• NCBI Official Full Name: POZ-, AT hook-, and zinc finger-containing protein 1 long C isoform
• NCBI Official Synonym Full Names: POZ/BTB and AT hook containing zinc finger 1
• NCBI Official Symbol: PATZ1
• NCBI Official Synonym Symbols: ZSG; MAZR; PATZ; RIAZ; ZBTB19; ZNF278; dJ400N23
• NCBI Protein Information: POZ-, AT hook-, and zinc finger-containing protein 1
• UniProt Protein Name: POZ-, AT hook-, and zinc finger-containing protein 1
• Protein Family: POZ-, AT hook-, and zinc finger-containing protein
• UniProt Gene Name: PATZ1
• UniProt Synonym Gene Names: PATZ; RIAZ; ZBTB19; ZNF278; ZSG
• UniProt Entry Name: PATZ1_HUMAN

NCBI Description

The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PATZ1: Transcriptional repressor. A chromosomal aberration involving PATZ1 is associated with small round cell sarcoma. Translocation t(1;22)(p36.1;q12) with EWSR1. Belongs to the krueppel C2H2-type zinc-finger protein family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding; metal ion binding

Biological Process: male gonad development; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; spermatogenesis; T cell differentiation; transcription from RNA polymerase II promoter