Rabbit anti-Human CD22 Polyclonal Antibody | anti-CD22 antibody

CD22 (Phospho Tyr807) Polyclonal Antibody

Cat. Num. AAA9459165

• Gene Names: CD22; SIGLEC2; SIGLEC-2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CD22; Polyclonal Antibody; CD22 (Phospho Tyr807) Polyclonal Antibody; SIGLEC2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD antigen CD22; anti-CD22 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Phospho-CD22 (Y807) Polyclonal Antibody detects endogenous levels of CD22 protein only when phosphorylated at Y807.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD22 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin, section), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; IHC: 1/100-1/300; ELISA: 1/40000
• Immunogen: The antiserum was produced against synthesized peptide derived from human BL-CAM around the phosphorylation site of Tyr807. AA range:776-825
• Domain: Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
• Online Information: Siglec-2, Siglec-2 [3 Fc Domains]
• PTM: Phosphorylated on tyrosine residues by LYN.; Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
• Similarity: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family, Contains 1 Ig-like V-type (immunoglobulin-like) domain, Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
• Subunit: Predominantly monomer of isoform CD22-beta. Also found as heterodimer of isoform CD22-beta and a shorter isoform. Interacts with PTPN6/SHP-1, LYN, SYK, PIK3R1/PIK3R2 and PLCG1 upon phosphorylation. Interacts with GRB2, INPP5D and SHC1 upon phosphorylation (By similarity). May form a complex with INPP5D/SHIP, GRB2 and SHC1.
• !Tissue Specificity: B-lymphocytes.
• Alternative Products: Additional isoforms seem to exist
• Preparation and Storage: Store at -20 degree C for 1 year

Related Product Information for anti-CD22 antibody

Function: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Product Categories/Family for anti-CD22 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 297374825
• NCBI GeneID: 933
• NCBI Accession #: NP_001172028.1
• NCBI GenBank Nucleotide #: NM_001185099.1
• UniProt Accession #: P20273; O95699; O95701; O95702; O95703; Q01665; Q92872; Q92873; Q9UQA6; Q9UQA7; Q9UQA8; F5H7U3
• Molecular Weight: 95,348 Da
• NCBI Official Full Name: B-cell receptor CD22 isoform 2
• NCBI Official Synonym Full Names: CD22 molecule
• NCBI Official Symbol: CD22
• NCBI Official Synonym Symbols: SIGLEC2; SIGLEC-2
• NCBI Protein Information: B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2
• UniProt Protein Name: B-cell receptor CD22
• Protein Family: CD226 antigen
• UniProt Gene Name: CD22
• UniProt Synonym Gene Names: SIGLEC2; BL-CAM; Siglec-2
• UniProt Entry Name: CD22_HUMAN

Uniprot Description

CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to plasma membrane; external side of plasma membrane

Molecular Function: protein binding; carbohydrate binding

Biological Process: cell adhesion