Rat Caspase-12 ELISA Kit | Casp12 elisa kit

Rat Caspase-12 ELISA Kit

Cat. Num. AAA9425961

• Reactivity: Rat
• Synonyms: Caspase-12; Rat Caspase-12 ELISA Kit; CASP-12; Casp12; 3.4.22.-; Casp12 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat CASP-12. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 0.15 ng/mL -10.0 ng/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Casp12 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat caspase-12,CASP-12 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP-12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CASP-12 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CASP-12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CASP-12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18426852
• NCBI GeneID: 156117
• NCBI Accession #: NP_569106.1
• NCBI GenBank Nucleotide #: NM_130422.1
• UniProt Accession #: Q920D5
• Molecular Weight: 47,837 Da
• NCBI Official Full Name: caspase-12
• NCBI Official Synonym Full Names: caspase 12
• NCBI Official Symbol: Casp12
• NCBI Protein Information: caspase-12
• UniProt Protein Name: Caspase-12
• Protein Family: Caspase
• UniProt Gene Name: Casp12
• UniProt Synonym Gene Names: CASP-12

NCBI Description

cysteine-aspartic acid protease (caspase); involved with the terminal stage of apoptosis [RGD, Feb 2006]

Uniprot Description

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.-; Protease

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: cytosol; endoplasmic reticulum; intracellular membrane-bound organelle; nucleus

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; protease binding; protein binding

Biological Process: aging; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; protein autoprocessing; proteolysis; regulation of inflammatory response; response to anoxia; response to cold; response to drug; response to ethanol; response to inorganic substance; response to iron(III) ion; response to nicotine; response to organic nitrogen; unfolded protein response