Typical Testing Data/Standard Curve (for reference only)
Rabbit CASP12 ELISA Kit | Casp12 elisa kit
Rabbit CASP12 (Caspase 12) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Casp12. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Casp12 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Casp12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Casp12. You can calculate the concentration of Casp12 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
cysteine-aspartic acid protease (caspase); involved with the terminal stage of apoptosis [RGD, Feb 2006]
Uniprot Description
CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.22.-
Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; intracellular; cytosol; nucleus
Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity
Biological Process: response to drug; response to nicotine; response to inorganic substance; response to anoxia; response to iron(III) ion; positive regulation of apoptosis; unfolded protein response; apoptosis; positive regulation of caspase activity; response to organic nitrogen; proteolysis; regulation of apoptosis; protein autoprocessing; response to ethanol; regulation of inflammatory response; response to cold; aging
Research Articles on Casp12
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Product Notes
The Rabbit Casp12 casp12 (Catalog #AAA2511789) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2511789 ELISA Kit recognizes Rabbit Casp12. It is sometimes possible for the material contained within the vial of "CASP12, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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