Chicken CTX-2 ELISA Kit | CTX-2 elisa kit

Chicken CTX-2 ELISA Kit

Cat. Num. AAA266150

• Reactivity: Chicken
• Synonyms: CTX-2; Chicken CTX-2 ELISA Kit; CTX-2 elisa kit

• Reactivity: Chicken
• Sequence Length: 81

• Samples: Chicken serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTX-2 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Chicken CTX-II monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 2507397
• Molecular Weight: 9,041 Da
• NCBI Official Full Name: Cytotoxin 2
• UniProt Protein Name: Cytotoxin 2
• UniProt Gene Name: CX2
• UniProt Synonym Gene Names: CTX II; CTX-2; CTX-A2; CTX2
• UniProt Entry Name: CTXA2_NAJAT

Uniprot Description

Basic protein that binds to cell membrane and depolarizes cardiomyocytes. It also shows lytic activities, but 2-fold less important than that of CTX-A4. It binds to the integrin alpha-V/beta-3 (ITGAV/ITGB3) with a moderate affinity. It may interact with sulfatides in the cell membrane which induces pore formation and cell internalization and is responsible for cytotoxicity in cardiomyocytes. It also may target the mitochondrial membrane and induce mitochondrial swelling and fragmentation.