Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-3: Jurkat, 293T and MCF7 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second)
Rabbit anti-Human ATP5A1 Polyclonal Antibody | anti-ATP5A1 antibody
ATP5A1 Antibody
Immunohistochemistry: 1:25-1:100
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-3: Jurkat, 293T and MCF7 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second)
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-3: Jurkat, 293T and MCF7 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/20.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/20.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.)
NCBI and Uniprot Product Information
Uniprot Description
ATP5A1: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Belongs to the ATPase alpha/beta chains family.
Protein type: EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase; Membrane protein, integral; Mitochondrial; Transporter
Chromosomal Location of Human Ortholog: 18q21
Cellular Component: extracellular matrix; membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex; mitochondrion; plasma membrane; signalosome
Molecular Function: ATP binding; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; MHC class I protein binding; protein binding; transmembrane transporter activity
Biological Process: ATP biosynthetic process; embryonic development; lipid metabolic process; mitochondrial ATP synthesis coupled proton transport; negative regulation of endothelial cell proliferation
Disease: Combined Oxidative Phosphorylation Deficiency 22; Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 4
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Product Notes
The ATP5A1 atp5a1 (Catalog #AAA9415712) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP5A1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the ATP5A1 atp5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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