Rabbit anti-Human ATP5A1 Polyclonal Antibody | anti-ATP5A1 antibody

ATP5A1 Antibody

Cat. Num. AAA7005662

• Gene Names: ATP5A1; OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: ATP5A1; Polyclonal Antibody; ATP5A1 Antibody; ATP synthase subunit alpha; mitochondrial; ATP5A; ATP5AL2; ATPM; anti-ATP5A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 503

• Applicable Applications for anti-ATP5A1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilution: WB:1:500-2000
• Immunogen: Recombinant human ATP synthase subunit alpha, mitochondrial protein(44-553AA)
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49162 / sc-49164 / sc-134728
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: ATP5A1 antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:COLO205 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 60,55,58 kDaObserved band size: 60 kDa)

Related Product Information for anti-ATP5A1 antibody

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.

NCBI and Uniprot Product Information

• NCBI GI #: 50345982
• NCBI GeneID: 498
• NCBI Accession #: NP_001001935.1
• NCBI GenBank Nucleotide #: NM_001001935.2
• UniProt Accession #: P25705; Q53XX6; Q8IXV2; Q96FB4; Q96HW2; Q96IR6; Q9BTV8; A8K092; B4DY56; K7ENP3
• Molecular Weight: 57,547 Da
• NCBI Official Full Name: ATP synthase subunit alpha, mitochondrial isoform c
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• NCBI Official Symbol: ATP5A1
• NCBI Official Synonym Symbols: OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
• NCBI Protein Information: ATP synthase subunit alpha, mitochondrial
• UniProt Protein Name: ATP synthase subunit alpha, mitochondrial
• UniProt Gene Name: ATP5A1
• UniProt Synonym Gene Names: ATP5A; ATP5AL2; ATPM
• UniProt Entry Name: ATPA_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]

Uniprot Description

ATP5A1: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Belongs to the ATPase alpha/beta chains family.

Protein type: Transporter; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Membrane protein, integral; Hydrolase; Mitochondrial

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex; mitochondrion; myelin sheath; plasma membrane; proton-transporting ATP synthase complex, catalytic core F(1); signalosome

Molecular Function: ATP binding; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen ion transporting ATPase activity, rotational mechanism; MHC class I protein binding; protein binding; transmembrane transporter activity

Biological Process: ATP biosynthetic process; ATP hydrolysis coupled proton transport; cellular metabolic process; embryonic development; lipid metabolic process; mitochondrial ATP synthesis coupled proton transport; negative regulation of endothelial cell proliferation

Disease: Combined Oxidative Phosphorylation Deficiency 22; Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 4