Testing Data
(Sample (30 ug of whole cell lysate)A: Hep G2 12% SDS PAGEPrimary antibody diluted at 1: 1000)
Rabbit anti-Human DAP13 Polyclonal Antibody | anti-DAP13 antibody
DAP13 antibody
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200
Testing Data
(Sample (30 ug of whole cell lysate)A: Hep G2 12% SDS PAGEPrimary antibody diluted at 1: 1000)
Testing Data
(Sample (30 ug of whole cell lysate)A: Hep G2 12% SDS PAGEPrimary antibody diluted at 1: 1000)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA12 antibody at 1: 500 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA12 antibody at 1: 500 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12 antibody at 1: 200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12 antibody at 1: 200 dilution.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]
Uniprot Description
NDUFA12: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFA12 are the cause of Leigh syndrome (LS). An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia. Belongs to the complex I NDUFA12 subunit family.
Protein type: Mitochondrial; Oxidoreductase; EC 1.6.99.3; EC 1.6.5.3
Chromosomal Location of Human Ortholog: 12q22
Cellular Component: mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial respiratory chain complex I
Molecular Function: NADH dehydrogenase (ubiquinone) activity; electron carrier activity
Biological Process: cellular metabolic process; respiratory gaseous exchange; response to oxidative stress
Disease: Leigh Syndrome
Research Articles on DAP13
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Product Notes
The DAP13 ndufa12 (Catalog #AAA9411877) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAP13 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DAP13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:50-1:500 Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the DAP13 ndufa12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAP13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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