Mouse anti-Human APC Monoclonal Antibody | anti-APC antibody

APC (Adenomatous Polyposis Coli Protein, Protein APC, Deleted in Polyposis 2.5, DP2.5) (FITC)

Cat. Num. AAA6145926

• Gene Names: APC; GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: APC; Monoclonal Antibody; APC (Adenomatous Polyposis Coli Protein; Protein APC; Deleted in Polyposis 2.5; DP2.5) (FITC); anti-APC antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3E2
• Specificity: Recognizes human APC.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 2843

• Applicable Applications for anti-APC antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant protein corresponding to aa2744-2843 from human APC with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: NPVPVSETNESSIVERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVNNNTKKRDSKTDSTESSGTQSPKRHSGSYLVTSV
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Immunofluorescence on HeLa cells using 123391 (10ug/ml))

Related Product Information for anti-APC antibody

Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Product Categories/Family for anti-APC antibody

Antibodies; Tumor Suppressors

NCBI and Uniprot Product Information

• NCBI GI #: 53759122
• NCBI GeneID: 324
• NCBI Accession #: NP_000029
• NCBI GenBank Nucleotide #: NM_000038
• UniProt Accession #: P25054
• NCBI Official Full Name: adenomatous polyposis coli protein isoform b
• NCBI Official Synonym Full Names: APC regulator of WNT signaling pathway
• NCBI Official Symbol: APC
• NCBI Official Synonym Symbols: GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46
• NCBI Protein Information: adenomatous polyposis coli protein
• UniProt Protein Name: Adenomatous polyposis coli protein
• Protein Family: APC-related protein
• UniProt Gene Name: APC
• UniProt Synonym Gene Names: DP2.5; Protein APC
• UniProt Entry Name: APC_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Mutations in the APC gene have been found to occur in most colorectal cancers. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Dec 2019]

Uniprot Description

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: centrosome; tight junction; cytosol; beta-catenin destruction complex; nucleoplasm; kinetochore; cell-cell adherens junction; lamellipodium; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; gamma-catenin binding; microtubule binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: positive regulation of cell adhesion; somatic stem cell maintenance; apoptosis; positive regulation of apoptosis; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; muscle maintenance; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of osteoclast differentiation; hair follicle development; cytokinesis after mitosis; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; cell adhesion; cell cycle arrest; kidney development; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; regulation of attachment of spindle microtubules to kinetochore; negative regulation of odontogenesis; cell migration; thymus development; regulation of osteoblast differentiation; negative regulation of MAPKKK cascade; negative regulation of microtubule depolymerization; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; axonogenesis; dorsal/ventral pattern formation; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration

Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma