Rabbit anti-Human NANS Polyclonal Antibody | anti-NANS antibody

NANS antibody

Cat. Num. AAA9408934

• Gene Names: NANS; SAS; HEL-S-100
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: NANS; Polyclonal Antibody; NANS antibody; SAS; anti-NANS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 359

• Applicable Applications for anti-NANS antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:250
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 54 and 341 of NANS
• Target Name: NANS
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE NANS antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded normal gastric epithelium (gland), using NANS antibody at 1: 100 dilution.)

Related Product Information for anti-NANS antibody

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq]

Product Categories/Family for anti-NANS antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 12056473
• NCBI GeneID: 54187
• NCBI Accession #: NP_061819
• NCBI GenBank Nucleotide #: NM_018946.3
• Molecular Weight: 40,308 Da
• NCBI Official Full Name: sialic acid synthase
• NCBI Official Synonym Full Names: N-acetylneuraminic acid synthase
• NCBI Official Symbol: NANS
• NCBI Official Synonym Symbols: SAS; HEL-S-100
• NCBI Protein Information: sialic acid synthase
• UniProt Protein Name: Sialic acid synthase
• Protein Family: Sialic acid synthase
• UniProt Gene Name: NANS
• UniProt Synonym Gene Names: SAS
• UniProt Entry Name: SIAS_HUMAN

NCBI Description

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]

Uniprot Description

NANS: Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3- deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N- acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.

Protein type: Transferase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.5.1.57; EC 2.5.1.56

Chromosomal Location of Human Ortholog: 9p24.1-p23

Cellular Component: cytoplasm; cytosol

Molecular Function: N-acylneuraminate-9-phosphate synthase activity; N-acetylneuraminate synthase activity; N-acylneuraminate cytidylyltransferase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; lipopolysaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification