Testing Data
(Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000)
Rabbit anti-Human, Mouse ACAT1 Polyclonal Antibody | anti-ACAT1 antibody
ACAT1 Antibody
Immunofluorescence: 1:100-1:1000
Testing Data
(Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000)
Testing Data
(Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000)
Testing Data
(Sample(30 mug of whole cell lysate)A:293TB:A431C:MOLT410% SDS PAGE diluted at 1:500)
Testing Data
(Sample(30 mug of whole cell lysate)A:293TB:A431C:MOLT410% SDS PAGE diluted at 1:500)
Immunofluorescence (IF)
(ACAT1 antibody detects ACAT1 protein at mitochondria by immunofluorescent analysis.Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37˚C for 30 min.Green: ACAT1 protein stained by ACAT1 antibody () diluted at 1:500.Blue: Hoechst 33342 staining.)
Immunofluorescence (IF)
(ACAT1 antibody detects ACAT1 protein at mitochondria by immunofluorescent analysis.Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37˚C for 30 min.Green: ACAT1 protein stained by ACAT1 antibody () diluted at 1:500.Blue: Hoechst 33342 staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
Uniprot Description
ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.
Protein type: Acetyltransferase; Carbohydrate Metabolism - propanoate; Mitochondrial; EC 2.3.1.9; Amino Acid Metabolism - tryptophan; Lipid Metabolism - synthesis and degradation of ketone bodies; Lipid Metabolism - fatty acid; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - pyruvate
Chromosomal Location of Human Ortholog: 11q22.3
Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane
Molecular Function: enzyme binding; protein homodimerization activity; acetyl-CoA C-acetyltransferase activity; metal ion binding; coenzyme binding
Biological Process: response to starvation; ketone body catabolic process; response to hormone stimulus; ketone body biosynthetic process; ketone body metabolic process; cellular lipid metabolic process; branched chain family amino acid catabolic process; brain development; liver development; response to organic cyclic substance; protein homooligomerization
Disease: Alpha-methylacetoacetic Aciduria
Research Articles on ACAT1
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Product Notes
The ACAT1 acat1 (Catalog #AAA9408095) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACAT1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ACAT1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). Western blotting: 1:500-1:3000 Immunofluorescence: 1:100-1:1000. Researchers should empirically determine the suitability of the ACAT1 acat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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