Rabbit TOLLIP Polyclonal Antibody | anti-TOLLIP antibody

TOLLIP Antibody

Cat. Num. AAA9406195

• Gene Names: TOLLIP; IL-1RAcPIP
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: TOLLIP; Polyclonal Antibody; TOLLIP Antibody; Toll interacting protein; anti-TOLLIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 274

• Applicable Applications for anti-TOLLIP antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 16 amino acid peptide from near the center of human TOLLIP.
• Target Name: TOLLIP
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 0.5, (B) 1 and (C) 2ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of TOLLIP in THP-1 cells with TOLLIP antibody at 2 ug/mL.)

Related Product Information for anti-TOLLIP antibody

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include TOLLIP, MyD88, and TRIF. TOLLIP associates directly with TLR2 and TLR 4, acting as an inhibitor to TLR activation. This negative regulation of TLR signaling may serve to limit the production of proinflammatory mediators during infection and inflammation.

Product Categories/Family for anti-TOLLIP antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 17390641
• NCBI GeneID: 54472
• Molecular Weight: 22,812 Da
• NCBI Official Full Name: Toll interacting protein
• NCBI Official Synonym Full Names: toll interacting protein
• NCBI Official Symbol: TOLLIP
• NCBI Official Synonym Symbols: IL-1RAcPIP
• NCBI Protein Information: toll-interacting protein
• UniProt Protein Name: Toll-interacting protein
• Protein Family: Toll-interacting protein
• UniProt Gene Name: TOLLIP
• UniProt Entry Name: TOLIP_HUMAN

NCBI Description

This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. [provided by RefSeq, Aug 2011]

Uniprot Description

TOLLIP: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2. Belongs to the tollip family.

Protein type: Vesicle; Inhibitor

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nuclear body; interleukin-1 receptor complex; perinuclear region of cytoplasm; cytoplasm; interleukin-18 receptor complex; cytosol

Molecular Function: signal transducer activity; protein binding; SUMO binding; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; interleukin-1, Type I receptor binding; kinase binding

Biological Process: positive regulation of protein sumoylation; epithelial cell differentiation; cell-cell signaling; autophagy; innate immune response; signal transduction; inflammatory response; phosphorylation; leukocyte activation