TOLLIP Antibody | anti-TOLLIP antibody

TOLLIP Antibody

Cat. Num. AAA8516395

• Gene Names: TOLLIP; IL-1RAcPIP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified
• Synonyms: TOLLIP; TOLLIP Antibody; FLJ33531; IL-1RAcPIP; anti-TOLLIP antibody

• Reactivity: Human, Mouse, Rat
• Specificity: TOLLIP Antibody detects endogenous levels of total TOLLIP
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 224

• Applicable Applications for anti-TOLLIP antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human TOLLIP
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts of PC-3cell lines, using TOLLIP antibody.)

Related Product Information for anti-TOLLIP antibody

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-kappaB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IkappaB, which normally maintains NF-kappaB in an inactive state by sequestering it in the cytoplasm. Tollip (Toll interacting protein) is an adaptor protein discovered to be associated with the IRAK complex and recruited to IL1-R following IL-1 stimulation (4). Overexpression of Tollip results in impaired NF-kappaB signaling (4). Tollip also associates directly with TLR2 and TLR4 and inhibits TLR-mediated signaling through inhibition of IRAK (5). Studies of Tollip deficient mice suggest that it plays a role in the regulation of inflammatory cytokines in response to IL-1 and LPS (6).

NCBI and Uniprot Product Information

• NCBI GI #: 972781729
• NCBI GeneID: 54472
• NCBI Accession #: NP_001305441.1
• NCBI GenBank Nucleotide #: NM_001318512.1
• UniProt Accession #: Q9H0E2; Q9H9E6; Q9UJ69; B3KXC6
• Molecular Weight: 30kDa
• NCBI Official Full Name: toll-interacting protein isoform 2
• NCBI Official Synonym Full Names: toll interacting protein
• NCBI Official Symbol: TOLLIP
• NCBI Official Synonym Symbols: IL-1RAcPIP
• NCBI Protein Information: toll-interacting protein
• UniProt Protein Name: Toll-interacting protein
• Protein Family: Toll-interacting protein
• UniProt Gene Name: TOLLIP

NCBI Description

This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity (PubMed:11751856). Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).