Rabbit anti-Human Tollip Polyclonal Antibody | anti-TOLLIP antibody

Anti-Tollip antibody

Cat. Num. AAA176316

• Gene Names: TOLLIP; IL-1RAcPIP
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Tollip; Polyclonal Antibody; Anti-Tollip antibody; Toll-interacting protein; toll interacting protein; Adapter protein antibody; FLJ33531 antibody; IL 1RAcPIP antibody; IL1RAcPIP antibody; TOLIP antibody; TOLIP_HUMAN antibody; Toll interacting protein antibody; Toll-interacting protein antibody; tollip antibody; anti-TOLLIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 274

• Applicable Applications for anti-TOLLIP antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Tollip (224-243aa NAQPRCSEEDLKAIQDMFPN).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Tollip antibody, MBS176316, Western blottingLane 1: PANC Cell LysateLane 2: HELA Cell LysateLane 3: U87 Cell Lysate )

Immunohistochemistry (IHC)

(Anti-Tollip antibody, MBS176316, IHC(P)IHC(P): Human Prostatic Cancer Tissue )

Related Product Information for anti-TOLLIP antibody

Description: Rabbit IgG polyclonal antibody for Toll-interacting protein(TOLLIP) detection. Tested with WB, IHC-P in Human.
Background: TOLLIP(TOLL-Interacting Protein), is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene. Lo et al.(2009) stated that the TOLLIP gene maps to chromosome 11. The mouse gene maps to chromosome 7. By Western blot analysis of embryonic kidney cells, Burns et al.(2000) confirmed the binding of TOLLIP to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. Burns et al.(2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by TOLLIP-IRAK complexes, and the phosphorylation of IRAK by MYD88. This leads to the dissociation of TOLLIP from IRAK, which can then transmit the IL1-induced signals.

NCBI and Uniprot Product Information

• NCBI GI #: 20140803
• NCBI GeneID: 54472
• UniProt Accession #: Q9H0E2; Q9H9E6; Q9UJ69; B3KXC6
• Molecular Weight: 22,812 Da
• NCBI Official Full Name: Toll-interacting protein
• NCBI Official Synonym Full Names: toll interacting protein
• NCBI Official Symbol: TOLLIP
• NCBI Official Synonym Symbols: IL-1RAcPIP
• NCBI Protein Information: toll-interacting protein; adapter protein
• UniProt Protein Name: Toll-interacting protein
• Protein Family: Toll-interacting protein
• UniProt Gene Name: TOLLIP
• UniProt Entry Name: TOLIP_HUMAN

NCBI Description

This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. [provided by RefSeq, Aug 2011]

Uniprot Description

TOLLIP: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2. Belongs to the tollip family.

Protein type: Inhibitor; Vesicle

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nuclear body; interleukin-1 receptor complex; perinuclear region of cytoplasm; cytoplasm; interleukin-18 receptor complex; cytosol

Molecular Function: protein binding; signal transducer activity; SUMO binding; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; interleukin-1, Type I receptor binding; kinase binding

Biological Process: positive regulation of protein sumoylation; cell-cell signaling; epithelial cell differentiation; autophagy; innate immune response; inflammatory response; signal transduction; phosphorylation; leukocyte activation