Mouse CD70 molecule ELISA Kit | CD70 elisa kit

Mouse CD70 antigen, CD70 ELISA Kit

Cat. Num. AAA927396

• Gene Names: Cd70; Cd27l; CD27LG; Tnfsf7
• Reactivity: Mouse
• Synonyms: CD70 molecule; Mouse CD70 antigen; CD70 ELISA Kit; Mouse CD70 antigen (CD70) ELISA kit; CD27L; CD27LG; TNFSF7; CD27 ligand; CD70 antigen; Ki-24 antigen; surface antigen CD70; tumor necrosis factor (ligand) superfamily; member 7; tumor necrosis factor ligand superfamily; CD70 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse CD70. No significant cross-reactivity or interference between mouse CD70 and analogues was observed.
• Sequence Length: 195

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml.
• Sensitivity: The minimum detectable dose of mouse CD70 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for CD70 elisa kit

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD70 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD70 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6755839
• NCBI GeneID: 21948
• NCBI Accession #: NP_035747.1
• NCBI GenBank Nucleotide #: NM_011617.2
• UniProt Accession #: O55237
• Molecular Weight: 21,919 Da
• NCBI Official Full Name: CD70 antigen
• NCBI Official Synonym Full Names: CD70 antigen
• NCBI Official Symbol: Cd70
• NCBI Official Synonym Symbols: Cd27l; CD27LG; Tnfsf7
• NCBI Protein Information: CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7
• UniProt Protein Name: CD70 antigen
• Protein Family: CD70 antigen
• UniProt Gene Name: Cd70
• UniProt Synonym Gene Names: Cd27l; Cd27lg; Tnfsf7; CD27-L
• UniProt Entry Name: CD70_MOUSE

Uniprot Description

CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Membrane protein, integral

Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane

Molecular Function: protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction